Induction of skin allograft transplantation tolerance in mice using human adipose derived stromal cells

Anthony D. Foster, Nicholas Clark, Thomas A. Davis*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Murine models of allograft transplantation are valuable for understanding the immunological mechanisms of allograft acceptance and rejection, the evaluation of immunosuppressive drugs and strategies, and the restoration of functional defects. Herein, we describe methods to create a skin murine allograft surgical model and how to administer adipose-derived stromal cells (ASC) with limited numbers of donor bone marrow to create stable multilineage donor cell chimerism and indefinite immunological tolerance.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages73-91
Number of pages19
DOIs
StatePublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1773
ISSN (Print)1064-3745

Keywords

  • Adipose stem cells
  • Donor cell chimerism
  • In vivo immune regulation
  • Skin allograft transplantation
  • Tolerance induction

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