Infectious virion capture by HIV-1 gp120-specific IgG from RV144 vaccinees

Pinghuang Liu, Nicole L. Yates, Xiaoying Shen, Mattia Bonsignori, M. Anthony Moody, Hua Xin Liao, Youyi Fong, S. Munir Alam, R. Glenn Overman, Thomas Denny, Guido Ferrari, Christina Ochsenbauer, John C. Kappes, Victoria R. Polonis, Punnee Pitisuttithum, Jaranit Kaewkungwal, Sorachai Nitayaphan, Supachai Rerks-Ngarm, David C. Montefiori, Peter GilbertNelson L. Michael, Jerome H. Kim, Barton F. Haynes, Georgia D. Tomaras*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

The detailed examination of the antibody repertoire from RV144 provides a unique template for understanding potentially protective antibody functions. Some potential immune correlates of protection were untested in the correlates analyses due to inherent assay limitations, as well as the need to keep the correlates analysis focused on a limited number of endpoints to achieve statistical power. In an RV144 pilot study, we determined that RV144 vaccination elicited antibodies that could bind infectious virions (including the vaccine strains HIV-1 CM244 and HIV-1MNand an HIV-1 strain expressing transmitted/founder Env, B.WITO.c). Among vaccinees with the highest IgG binding antibody profile, the majority (78%) captured the infectious vaccine strain virus (CM244), while a smaller proportion of vaccinees (26%) captured HIV-1 transmitted/founder Env virus. We demonstrated that vaccine-elicited HIV-1 gp120 antibodies of multiple specificities (V3, V2, conformational C1, and gp120 conformational) mediated capture of infectious virions. Although capture of infectious HIV-1 correlated with other humoral immune responses, the extent of variation between these humoral responses and virion capture indicates that virion capture antibodies occupy unique immunological space.

Original languageEnglish
Pages (from-to)7828-7836
Number of pages9
JournalJournal of Virology
Volume87
Issue number14
DOIs
StatePublished - Jul 2013
Externally publishedYes

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