Innate immune responses in swine resuscitated from severe traumatic hemorrhagic shock with hemoglobin-based oxygen carrier-201

C. Hall, N. Malkevich*, M. Handrigan, C. VanderMolen, F. Aranaud, J. Hong, F. Dong, J. Rice, N. Philbin, S. Ahlers, R. McCarron, D. Freilich, G. McGwin, W. S. Flournoy, L. Bruce Pearce

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Hemoglobin-based oxygen carrier-201 transports oxygen and improves survival in swine with hemorrhagic shock, but has potential to be immune activating. Herein, we evaluated HBOC-201's immune effects in swine with more severe hemorrhagic shock due to soft tissue injury and 55% blood volume catheter withdrawal over 15 minutes followed by fluid resuscitation at 20 minutes with HBOC-201, Hextend, or no treatment (NON) before hospital arrival. Survival rates were similar with HBOC-201 and Hextend (p > 0.05), but were higher than in (p = 0.007). There were no significant group differences in blood cell count, percentages of leukocyte sub-populations and immunophenotype (CD4:CD8 ratio), adhesion markers expression (neutrophil CD11b; monocyte or neutrophil CD49d) and apoptosis. There was a trend to higher plasma IL-10 in HBOC-201 and groups vs. Hextend. We conclude that in swine with severe controlled HS and soft tissue injury, immune responses are similar with resuscitation with HBOC-201 and Hextend.

Original languageEnglish
Pages (from-to)259-274
Number of pages16
JournalArtificial Cells, Blood Substitutes, and Biotechnology
Volume35
Issue number3
DOIs
StatePublished - May 2007
Externally publishedYes

Keywords

  • Adhesion markers
  • Apoptosis
  • Cytokines
  • HBOC-201
  • Hemorrhagic shock
  • Hextend
  • Resuscitation
  • Trauma

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