Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis

Qingnian Goh, Christopher L. Dearth, Jacob T. Corbett, Philippe Pierre, Deborah N. Chadee, Francis X. Pizza*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We previously demonstrated that the expression of intercellular adhesion molecule-1 (ICAM-1) by skeletal muscle cells after muscle overload contributes to ensuing regenerative and hypertrophic processes in skeletal muscle. The objective of the present study is to reveal mechanisms through which skeletal muscle cell expression of ICAM-1 augments regenerative and hypertrophic processes of myogenesis. This was accomplished by genetically engineering C2C12 myoblasts to stably express ICAM-1, and by inhibiting the adhesive and signaling functions of ICAM-1 through the use of a neutralizing antibody or cell penetrating peptide, respectively. Expression of ICAM-1 by cultured skeletal muscle cells augmented myoblast-myoblast adhesion, myotube formation, myonuclear number, myotube alignment, myotube-myotube fusion, and myotube size without influencing the ability of myoblasts to proliferate or differentiate. ICAM-1 augmented myotube formation, myonuclear accretion, and myotube alignment through a mechanism involving adhesion-induced activation of ICAM-1 signaling, as these dependent measures were reduced via antibody and peptide inhibition of ICAM-1. The adhesive and signaling functions of ICAM-1 also facilitated myotube hypertrophy through a mechanism involving myotube-myotube fusion, protein synthesis, and Akt/p70s6k signaling. Our findings demonstrate that ICAM-1 expression by skeletal muscle cells augments myogenesis, and establish a novel mechanism through which the inflammatory response facilitates growth processes in skeletal muscle.

Original languageEnglish
Pages (from-to)292-308
Number of pages17
JournalExperimental Cell Research
Volume331
Issue number2
DOIs
StatePublished - 15 Feb 2015
Externally publishedYes

Keywords

  • Adhesion molecules
  • Inflammation
  • Muscle hypertrophy
  • Muscle regeneration

Fingerprint

Dive into the research topics of 'Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis'. Together they form a unique fingerprint.

Cite this