Interferon alters intracellular transport of vesicular stomatitis virus glycoprotein

Double-label immunofluorescence staining studies in virus-infected subclone 11 of L(B) cells indiated that almost all of the vesicular stomatitis virus (VSV) glycoprotein (G) was plasma membrane-associated during the logarithmic phase of virus replication. In contrast, treatment with interferon (IFN) resulted in inhibition of VSV-G transport, so that almost all of the G remained associated with the Golgi complex (GC) at comparable times after infection. In both IFN-treated and control cells, G was resistant to treatment with the enzyme endo-β-N-acetylglucosamine H (endo H) indicating that the bulk of the G had reached the trans compartment of the GC.