Isolation and Growth Kinetics of Bogoria Virus from Phlebotomine Sandflies Sampled in Baringo, Kenya

Jane Wambui Thiiru; Solomon Langat; Francis Mulwa; Stephanie Cinkovich; Hellen Koka; Santos Yalwala; Samoel Khamadi; Justus Onguso; Nicholas Odemba; Francis Ngere; Jaree Johnson; Timothy Egbo; Eric Garges; Elly Ojwang; Fredrick Eyase

doi:10.4269/ajtmh.24-0032

Isolation and Growth Kinetics of Bogoria Virus from Phlebotomine Sandflies Sampled in Baringo, Kenya

Jane Wambui Thiiru^*, Solomon Langat, Francis Mulwa, Stephanie Cinkovich, Hellen Koka, Santos Yalwala, Samoel Khamadi, Justus Onguso, Nicholas Odemba, Francis Ngere, Jaree Johnson, Timothy Egbo, Eric Garges, Elly Ojwang, Fredrick Eyase

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Phleboviruses are an emerging threat to public health. Recent surveillance efforts in Kenya have unveiled novel phleboviruses. Despite these efforts, there remain knowledge gaps. This study tested female sandflies from diverse ecological settings in Kenya for arboviruses. Sandfly pools were cultured in Vero-CCL cells. Pools showing reproducible cytopathic effects were subjected to next-generation sequencing, followed by phylogenetic analysis. In vitro, cell kinetics analysis was performed using both Vero-E6 cells and C6/36 mosquito cells. One pool from Baringo, Kenya, tested positive for Bogoria virus (BOGV). The BOGV genome clustered in a single clade with previously obtained BOGV genomes. No significant differences were observed between Vero and C6/36 cell growth kinetics. This study has confirmed the presence of BOGV among sandflies in Baringo Kenya and demonstrated growth in mosquito cells.

Original language	English
Pages (from-to)	412-415
Number of pages	4
Journal	American Journal of Tropical Medicine and Hygiene
Volume	111
Issue number	2
DOIs	https://doi.org/10.4269/ajtmh.24-0032
State	Published - Aug 2024
Externally published	Yes

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10.4269/ajtmh.24-0032

Cite this

Thiiru, J. W., Langat, S., Mulwa, F., Cinkovich, S., Koka, H., Yalwala, S., Khamadi, S., Onguso, J., Odemba, N., Ngere, F., Johnson, J., Egbo, T., Garges, E., Ojwang, E., & Eyase, F. (2024). Isolation and Growth Kinetics of Bogoria Virus from Phlebotomine Sandflies Sampled in Baringo, Kenya. American Journal of Tropical Medicine and Hygiene, 111(2), 412-415. https://doi.org/10.4269/ajtmh.24-0032

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title = "Isolation and Growth Kinetics of Bogoria Virus from Phlebotomine Sandflies Sampled in Baringo, Kenya",

abstract = "Phleboviruses are an emerging threat to public health. Recent surveillance efforts in Kenya have unveiled novel phleboviruses. Despite these efforts, there remain knowledge gaps. This study tested female sandflies from diverse ecological settings in Kenya for arboviruses. Sandfly pools were cultured in Vero-CCL cells. Pools showing reproducible cytopathic effects were subjected to next-generation sequencing, followed by phylogenetic analysis. In vitro, cell kinetics analysis was performed using both Vero-E6 cells and C6/36 mosquito cells. One pool from Baringo, Kenya, tested positive for Bogoria virus (BOGV). The BOGV genome clustered in a single clade with previously obtained BOGV genomes. No significant differences were observed between Vero and C6/36 cell growth kinetics. This study has confirmed the presence of BOGV among sandflies in Baringo Kenya and demonstrated growth in mosquito cells.",

author = "Thiiru, {Jane Wambui} and Solomon Langat and Francis Mulwa and Stephanie Cinkovich and Hellen Koka and Santos Yalwala and Samoel Khamadi and Justus Onguso and Nicholas Odemba and Francis Ngere and Jaree Johnson and Timothy Egbo and Eric Garges and Elly Ojwang and Fredrick Eyase",

note = "Publisher Copyright: Copyright {\textcopyright} 2024 American Society of Tropical Medicine and Hygiene.",

year = "2024",

month = aug,

doi = "10.4269/ajtmh.24-0032",

language = "English",

volume = "111",

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Thiiru, JW, Langat, S, Mulwa, F, Cinkovich, S, Koka, H, Yalwala, S, Khamadi, S, Onguso, J, Odemba, N, Ngere, F, Johnson, J, Egbo, T, Garges, E, Ojwang, E & Eyase, F 2024, 'Isolation and Growth Kinetics of Bogoria Virus from Phlebotomine Sandflies Sampled in Baringo, Kenya', American Journal of Tropical Medicine and Hygiene, vol. 111, no. 2, pp. 412-415. https://doi.org/10.4269/ajtmh.24-0032

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T1 - Isolation and Growth Kinetics of Bogoria Virus from Phlebotomine Sandflies Sampled in Baringo, Kenya

AU - Thiiru, Jane Wambui

AU - Langat, Solomon

AU - Mulwa, Francis

AU - Cinkovich, Stephanie

AU - Koka, Hellen

AU - Yalwala, Santos

AU - Khamadi, Samoel

AU - Onguso, Justus

AU - Odemba, Nicholas

AU - Ngere, Francis

AU - Johnson, Jaree

AU - Egbo, Timothy

AU - Garges, Eric

AU - Ojwang, Elly

AU - Eyase, Fredrick

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AB - Phleboviruses are an emerging threat to public health. Recent surveillance efforts in Kenya have unveiled novel phleboviruses. Despite these efforts, there remain knowledge gaps. This study tested female sandflies from diverse ecological settings in Kenya for arboviruses. Sandfly pools were cultured in Vero-CCL cells. Pools showing reproducible cytopathic effects were subjected to next-generation sequencing, followed by phylogenetic analysis. In vitro, cell kinetics analysis was performed using both Vero-E6 cells and C6/36 mosquito cells. One pool from Baringo, Kenya, tested positive for Bogoria virus (BOGV). The BOGV genome clustered in a single clade with previously obtained BOGV genomes. No significant differences were observed between Vero and C6/36 cell growth kinetics. This study has confirmed the presence of BOGV among sandflies in Baringo Kenya and demonstrated growth in mosquito cells.

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