Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa: Chromosomal mapping and expression

Arnould Savouré; Attila Fehér; Péter Kaló; György Petrovics; Gyula Csanádi; Judit Szecsi; György Kiss; Spencer Brown; Adam Kondorosi; Eva Kondorosi

doi:10.1007/BF00020880

Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa: Chromosomal mapping and expression

Arnould Savouré^*, Attila Fehér, Péter Kaló, György Petrovics, Gyula Csanádi, Judit Szecsi, György Kiss, Spencer Brown, Adam Kondorosi, Eva Kondorosi

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

Cyclins in association with the protein kinase p34^cdc2and related cyclin-dependent protein kinases (cdks) are key regulatory elements in controlling the cell division cycle. Here, we describe the identification and characterization of a full-length cDNA clone of alfalfa mitotic cyclin, termed CycIIIMs. Computer analysis of known plant cyclin gene sequences revealed that this cyclin belongs to the same structural group as the other known partial alfalfa cyclin sequences. Genetic segregation analysis based on DNA-DNA hybridization data showed that the CycIIIMs gene(s) locates in a single chromosomal region on linkage group 5 of the alfalfa genetic map between RFLP markers UO89A and CG13. The assignment of this cyclin to the mitotic cyclin class was based on its cDNA-derived sequence and its differential expression during G2/M cell cycle phase transition of a partially synchronized alfalfa cell culture. Sequence analysis indicated common motifs with both the A- and B-types of mitotic cyclins similarly to the newly described B3-type of animal cyclins.

Original language	English
Pages (from-to)	1059-1070
Number of pages	12
Journal	Plant Molecular Biology
Volume	27
Issue number	6
DOIs	https://doi.org/10.1007/BF00020880
State	Published - Mar 1995
Externally published	Yes

Keywords

Alfalfa
cell division cycle
chromosomal location
cyclin
gene expression

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title = "Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa: Chromosomal mapping and expression",

abstract = "Cyclins in association with the protein kinase p34cdc2and related cyclin-dependent protein kinases (cdks) are key regulatory elements in controlling the cell division cycle. Here, we describe the identification and characterization of a full-length cDNA clone of alfalfa mitotic cyclin, termed CycIIIMs. Computer analysis of known plant cyclin gene sequences revealed that this cyclin belongs to the same structural group as the other known partial alfalfa cyclin sequences. Genetic segregation analysis based on DNA-DNA hybridization data showed that the CycIIIMs gene(s) locates in a single chromosomal region on linkage group 5 of the alfalfa genetic map between RFLP markers UO89A and CG13. The assignment of this cyclin to the mitotic cyclin class was based on its cDNA-derived sequence and its differential expression during G2/M cell cycle phase transition of a partially synchronized alfalfa cell culture. Sequence analysis indicated common motifs with both the A- and B-types of mitotic cyclins similarly to the newly described B3-type of animal cyclins.",

keywords = "Alfalfa, cell division cycle, chromosomal location, cyclin, gene expression",

author = "Arnould Savour{\'e} and Attila Feh{\'e}r and P{\'e}ter Kal{\'o} and Gy{\"o}rgy Petrovics and Gyula Csan{\'a}di and Judit Szecsi and Gy{\"o}rgy Kiss and Spencer Brown and Adam Kondorosi and Eva Kondorosi",

year = "1995",

month = mar,

doi = "10.1007/BF00020880",

language = "English",

volume = "27",

pages = "1059--1070",

journal = "Plant Molecular Biology",

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T1 - Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa

T2 - Chromosomal mapping and expression

AU - Savouré, Arnould

AU - Fehér, Attila

AU - Kaló, Péter

AU - Petrovics, György

AU - Csanádi, Gyula

AU - Szecsi, Judit

AU - Kiss, György

AU - Brown, Spencer

AU - Kondorosi, Adam

AU - Kondorosi, Eva

PY - 1995/3

Y1 - 1995/3

N2 - Cyclins in association with the protein kinase p34cdc2and related cyclin-dependent protein kinases (cdks) are key regulatory elements in controlling the cell division cycle. Here, we describe the identification and characterization of a full-length cDNA clone of alfalfa mitotic cyclin, termed CycIIIMs. Computer analysis of known plant cyclin gene sequences revealed that this cyclin belongs to the same structural group as the other known partial alfalfa cyclin sequences. Genetic segregation analysis based on DNA-DNA hybridization data showed that the CycIIIMs gene(s) locates in a single chromosomal region on linkage group 5 of the alfalfa genetic map between RFLP markers UO89A and CG13. The assignment of this cyclin to the mitotic cyclin class was based on its cDNA-derived sequence and its differential expression during G2/M cell cycle phase transition of a partially synchronized alfalfa cell culture. Sequence analysis indicated common motifs with both the A- and B-types of mitotic cyclins similarly to the newly described B3-type of animal cyclins.

AB - Cyclins in association with the protein kinase p34cdc2and related cyclin-dependent protein kinases (cdks) are key regulatory elements in controlling the cell division cycle. Here, we describe the identification and characterization of a full-length cDNA clone of alfalfa mitotic cyclin, termed CycIIIMs. Computer analysis of known plant cyclin gene sequences revealed that this cyclin belongs to the same structural group as the other known partial alfalfa cyclin sequences. Genetic segregation analysis based on DNA-DNA hybridization data showed that the CycIIIMs gene(s) locates in a single chromosomal region on linkage group 5 of the alfalfa genetic map between RFLP markers UO89A and CG13. The assignment of this cyclin to the mitotic cyclin class was based on its cDNA-derived sequence and its differential expression during G2/M cell cycle phase transition of a partially synchronized alfalfa cell culture. Sequence analysis indicated common motifs with both the A- and B-types of mitotic cyclins similarly to the newly described B3-type of animal cyclins.

KW - Alfalfa

KW - cell division cycle

KW - chromosomal location

KW - cyclin

KW - gene expression

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U2 - 10.1007/BF00020880

DO - 10.1007/BF00020880

M3 - Article

C2 - 7766889

AN - SCOPUS:0029257558

SN - 0167-4412

VL - 27

SP - 1059

EP - 1070

JO - Plant Molecular Biology

JF - Plant Molecular Biology

IS - 6

ER -

Isolation of a full-length mitotic cyclin cDNA clone CycIIIMs from Medicago sativa: Chromosomal mapping and expression

Abstract

Keywords

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