TY - JOUR
T1 - Isolation of rsp-1, a Novel cDNA Capable of Suppressing v-Ras Transformation
AU - Cutler, M. L.
AU - Bassin, R. H.
AU - Zanoni, L.
AU - Talbot, N.
PY - 1992/9
Y1 - 1992/9
N2 - Using an expression cloning assay, we have isolated a novel cDNA, referred to as rsp-1, which suppresses the v-Ras-transformed phenotype. When introduced into NIH 3T3 fibroblasts under the control of a metallothionein promoter, rsp-1 confers resistance to v-Ras, but not to v-Mos or v-Src, and inhibits growth of the cells. The rsp-1 cDNA contains a 831-bp open reading frame encoding a 277-amino-acid leucine-rich protein. The rsp-1 cDNA exhibits no significant homology to sequences in the DNA data bases. However, searches of the protein data bases revealed that it contains a series of leucine-based repeats which are homologous to the leucine repeats found in the regulatory region of the yeast adenylyl cyclase. rsp-1 specific RNA is detectable in a wide variety of cell lines and tissues, and the gene is conserved among eukaryotic species. These data suggest that rsp-1 plays a role in Ras signal transduction.
AB - Using an expression cloning assay, we have isolated a novel cDNA, referred to as rsp-1, which suppresses the v-Ras-transformed phenotype. When introduced into NIH 3T3 fibroblasts under the control of a metallothionein promoter, rsp-1 confers resistance to v-Ras, but not to v-Mos or v-Src, and inhibits growth of the cells. The rsp-1 cDNA contains a 831-bp open reading frame encoding a 277-amino-acid leucine-rich protein. The rsp-1 cDNA exhibits no significant homology to sequences in the DNA data bases. However, searches of the protein data bases revealed that it contains a series of leucine-based repeats which are homologous to the leucine repeats found in the regulatory region of the yeast adenylyl cyclase. rsp-1 specific RNA is detectable in a wide variety of cell lines and tissues, and the gene is conserved among eukaryotic species. These data suggest that rsp-1 plays a role in Ras signal transduction.
UR - http://www.scopus.com/inward/record.url?scp=0026658729&partnerID=8YFLogxK
U2 - 10.1128/MCB.12.9.3750
DO - 10.1128/MCB.12.9.3750
M3 - Article
C2 - 1508180
AN - SCOPUS:0026658729
SN - 0270-7306
VL - 12
SP - 3750
EP - 3756
JO - Molecular and Cellular Biology
JF - Molecular and Cellular Biology
IS - 9
ER -