@article{28e41f9e11d5443c935ccdb5247bddb8,
title = "Isolation of YAC insert sequences by representational difference analysis",
abstract = "We present a method for the isolation of YAC insert sequences by representational difference analysis (RDA). To achieve maximal representation of the sequences, the amplicons were generated from a Mbol digestion product. RDA was performed using a 970 kb insert YAC clone. After two rounds of re-association and selective amplification 92% of the difference product represented sequences derived from the YAC insert. Twenty insert-specific sequence-tagged sites were readily defined. The difference product was also successfully used to isolate microsatellite markers, to identify clones from a human PAC library and as a chromosome painting probe in fluorescence in situ hybridization.",
author = "Mieke Schutte and {Da Costa}, {Luis T.} and Moskaluk, {Christopher A.} and Ester Rozenblum and Xiaoping Guan and {De Jong}, {Pieter J.} and Michael Bittner and Meltzer, {Paul S.} and Trent, {Jeffrey M.} and Kern, {Scott E.}",
note = "Funding Information: This work was supported by NIH SPORE grant in Gastrointestinal Cancer CA62924, by the Dutch Cancer Society KWF (MS) and by JNICT Scholarship BD1508/91-ID (LT da C). XG and PJ de J are supported by the Cancer Center Support grant at Roswell Park Cancer Institute CA-16056, by Department of Energy grant DE-FG02-94ER61883 and by NIH grant 1R01RG01165-01. SEK is a McDonnell Foundation Scholar.",
year = "1995",
month = oct,
day = "25",
doi = "10.1093/nar/23.20.4127",
language = "English",
volume = "23",
pages = "4127--4133",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "20",
}