Laser Microdissection for Gene Expression Profiling

Lori A. Field, Brenda Deyarmin, Craig D. Shriver, Darrell L. Ellsworth, Rachel E. Ellsworth*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

10 Scopus citations


Microarray-based gene expression profiling is revolutionizing biomedical research by allowing expression profiles of thousands of genes to be interrogated in a single experiment. In cancer research, the use of laser microdissection (LM) to isolate RNA from tissues provides the ability to accurately identify molecular profiles from different cell types that comprise the tumor and its surrounding microenvironment. Because RNA is an unstable molecule, the quality of RNA extracted from tissues can be affected by sample preparation and processing. Thus, special protocols have been developed to isolate research-quality RNA after LM. This chapter provides detailed descriptions of protocols used to generate micro­array data from high-quality frozen breast tissue specimens, as well as challenges associated with formalin-fixed paraffin-embedded specimens.

Original languageEnglish
Title of host publicationLaser Capture Microdissection
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages29
ISBN (Print)9781617791628
StatePublished - 2011
Externally publishedYes

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Breast cancer
  • FFPE
  • Frozen tissue
  • Gene expression
  • Laser microdissection
  • Microarray
  • Molecular signature


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