Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials

George S. Hussey; Catalina Pineda Molina; Madeline C. Cramer; Yulia Y. Tyurina; Vladimir A. Tyurin; Yoojin C. Lee; Salma O. El-Mossier; Mark H. Murdock; Peter S. Timashev; Valerian E. Kagan; Stephen F. Badylak

doi:10.1126/sciadv.aay4361

Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials

George S. Hussey, Catalina Pineda Molina, Madeline C. Cramer, Yulia Y. Tyurina, Vladimir A. Tyurin, Yoojin C. Lee, Salma O. El-Mossier, Mark H. Murdock, Peter S. Timashev, Valerian E. Kagan, Stephen F. Badylak^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM.

Original language	English
Article number	eaay4361
Journal	Science Advances
Volume	6
Issue number	12
DOIs	https://doi.org/10.1126/sciadv.aay4361
State	Published - 2020
Externally published	Yes

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Hussey, G. S., Molina, C. P., Cramer, M. C., Tyurina, Y. Y., Tyurin, V. A., Lee, Y. C., El-Mossier, S. O., Murdock, M. H., Timashev, P. S., Kagan, V. E., & Badylak, S. F. (2020). Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials. Science Advances, 6(12), Article eaay4361. https://doi.org/10.1126/sciadv.aay4361

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title = "Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials",

abstract = "Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM.",

author = "Hussey, {George S.} and Molina, {Catalina Pineda} and Cramer, {Madeline C.} and Tyurina, {Yulia Y.} and Tyurin, {Vladimir A.} and Lee, {Yoojin C.} and El-Mossier, {Salma O.} and Murdock, {Mark H.} and Timashev, {Peter S.} and Kagan, {Valerian E.} and Badylak, {Stephen F.}",

note = "Publisher Copyright: Copyright {\textcopyright} 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).",

year = "2020",

doi = "10.1126/sciadv.aay4361",

language = "English",

volume = "6",

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AU - Hussey, George S.

AU - Molina, Catalina Pineda

AU - Cramer, Madeline C.

AU - Tyurina, Yulia Y.

AU - Tyurin, Vladimir A.

AU - Lee, Yoojin C.

AU - El-Mossier, Salma O.

AU - Murdock, Mark H.

AU - Timashev, Peter S.

AU - Kagan, Valerian E.

AU - Badylak, Stephen F.

N1 - Publisher Copyright: Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

PY - 2020

Y1 - 2020

N2 - Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM.

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Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials

Abstract

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