@inbook{8c44c46d07bf4158adabd65fc4de8b17,
title = "Live-Cell Imaging of Dynein-Mediated Cargo Transport in Aspergillus nidulans",
abstract = "Filamentous fungi have been used for studying long-distance transport of cargoes driven by cytoplasmic dynein. Aspergillus nidulans is a well-established genetic model organism used for studying dynein function and regulation in vivo. Here, we describe how we grow A. nidulans strains for live-cell imaging and how we observe the dynein-mediated distribution of early endosomes and secretory vesicles. Using an on-stage incubator and culture chambers for inverted microscopes, we can image fungal hyphae that naturally attach to the bottom of the chambers, using wide-field epifluorescence microscopes or the new Zeiss LSM 980 (with Airyscan 2) microscope. In addition to methods for preparing cells for imaging, a procedure for A. nidulans transformation is also described.",
keywords = "Aspergillus nidulans, Dynein, Early endosomes, Filamentous fungi, Live-cell imaging, Secretory vesicles, Wide-field epifluorescence microscopes, Zeiss LSM 980 (with Airyscan 2)",
author = "Rongde Qiu and Jun Zhang and Dennis McDaniel and Pe{\~n}alva, {Miguel A.} and Xin Xiang",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2023",
doi = "10.1007/978-1-0716-2958-1_1",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "3--23",
booktitle = "Methods in Molecular Biology",
}