Loss of transcriptional repression contributes to the ectopic expression of the calcitonin/alpha-CGRP gene in a human lung carcinoma cell line

A J Symes; R K Craig; P M Brickell

doi:10.1016/0014-5793(92)81006-8

Loss of transcriptional repression contributes to the ectopic expression of the calcitonin/alpha-CGRP gene in a human lung carcinoma cell line

A J Symes, R K Craig, P M Brickell

Pharmacology and Molecular Therapeutics

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The calcitonin/alpha-CGRP (CT/CGRP) gene is ectopically expressed in a wide variety of neoplasia. We have investigated the molecular mechanisms responsible for this ectopic expression in the human cell line BEN, which is derived from a poorly differentiated squamous cell lung carcinoma. We show that a trans-acting factor which represses expression of the CT/CGRP gene in HeLa cells is absent or inactive in BEN cells, and have localised the repressor binding site to a 53 bp fragment 1500 bp upstream of the transcription start site.

Original language	English
Pages (from-to)	229-33
Number of pages	5
Journal	FEBS Letters
Volume	306
Issue number	2-3
DOIs	https://doi.org/10.1016/0014-5793(92)81006-8
State	Published - 20 Jul 1992

Keywords

Base Sequence
Binding Sites
Calcitonin Gene-Related Peptide/genetics
Carcinoma, Squamous Cell/genetics
DNA, Neoplasm/metabolism
Gene Expression Regulation, Neoplastic
HeLa Cells
Humans
Lung Neoplasms/genetics
Molecular Sequence Data
Repressor Proteins/metabolism
Restriction Mapping
Transcription, Genetic
Transfection
Tumor Cells, Cultured

Access to Document

10.1016/0014-5793(92)81006-8

Cite this

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title = "Loss of transcriptional repression contributes to the ectopic expression of the calcitonin/alpha-CGRP gene in a human lung carcinoma cell line",

abstract = "The calcitonin/alpha-CGRP (CT/CGRP) gene is ectopically expressed in a wide variety of neoplasia. We have investigated the molecular mechanisms responsible for this ectopic expression in the human cell line BEN, which is derived from a poorly differentiated squamous cell lung carcinoma. We show that a trans-acting factor which represses expression of the CT/CGRP gene in HeLa cells is absent or inactive in BEN cells, and have localised the repressor binding site to a 53 bp fragment 1500 bp upstream of the transcription start site.",

keywords = "Base Sequence, Binding Sites, Calcitonin Gene-Related Peptide/genetics, Carcinoma, Squamous Cell/genetics, DNA, Neoplasm/metabolism, Gene Expression Regulation, Neoplastic, HeLa Cells, Humans, Lung Neoplasms/genetics, Molecular Sequence Data, Repressor Proteins/metabolism, Restriction Mapping, Transcription, Genetic, Transfection, Tumor Cells, Cultured",

author = "Symes, {A J} and Craig, {R K} and Brickell, {P M}",

year = "1992",

month = jul,

day = "20",

doi = "10.1016/0014-5793(92)81006-8",

language = "English",

volume = "306",

pages = "229--33",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc",

number = "2-3",

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TY - JOUR

T1 - Loss of transcriptional repression contributes to the ectopic expression of the calcitonin/alpha-CGRP gene in a human lung carcinoma cell line

AU - Symes, A J

AU - Craig, R K

AU - Brickell, P M

PY - 1992/7/20

Y1 - 1992/7/20

N2 - The calcitonin/alpha-CGRP (CT/CGRP) gene is ectopically expressed in a wide variety of neoplasia. We have investigated the molecular mechanisms responsible for this ectopic expression in the human cell line BEN, which is derived from a poorly differentiated squamous cell lung carcinoma. We show that a trans-acting factor which represses expression of the CT/CGRP gene in HeLa cells is absent or inactive in BEN cells, and have localised the repressor binding site to a 53 bp fragment 1500 bp upstream of the transcription start site.

AB - The calcitonin/alpha-CGRP (CT/CGRP) gene is ectopically expressed in a wide variety of neoplasia. We have investigated the molecular mechanisms responsible for this ectopic expression in the human cell line BEN, which is derived from a poorly differentiated squamous cell lung carcinoma. We show that a trans-acting factor which represses expression of the CT/CGRP gene in HeLa cells is absent or inactive in BEN cells, and have localised the repressor binding site to a 53 bp fragment 1500 bp upstream of the transcription start site.

KW - Base Sequence

KW - Binding Sites

KW - Calcitonin Gene-Related Peptide/genetics

KW - Carcinoma, Squamous Cell/genetics

KW - DNA, Neoplasm/metabolism

KW - Gene Expression Regulation, Neoplastic

KW - HeLa Cells

KW - Humans

KW - Lung Neoplasms/genetics

KW - Molecular Sequence Data

KW - Repressor Proteins/metabolism

KW - Restriction Mapping

KW - Transcription, Genetic

KW - Transfection

KW - Tumor Cells, Cultured

U2 - 10.1016/0014-5793(92)81006-8

DO - 10.1016/0014-5793(92)81006-8

M3 - Article

C2 - 1633879

SN - 0014-5793

VL - 306

SP - 229

EP - 233

JO - FEBS Letters

JF - FEBS Letters

IS - 2-3

ER -