Mammalian liver contains an activity which mimics bacterial chloramphenicol acetyltransferase

Antonio De Maio*, Timothy G. Buchman

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Rat liver extracts contain an activity which mimics Escherichia coli chloramphenicol acetyltransferase (CAT); the latter is commonly used to report transcriptional activation of chimeric genes transfected into cultured cells. Although the activities are indistinguishable by the standard thin-layer chromatography assay, alternate methods can discriminate between them. The rat CAT-like activity appears to be an integral membrane protein. It was observed in the microsomal fraction of both liver and kidney. Similarly CAT-like activities were detected in mouse, rabbit and pig liver. In addition, liver homogenates which contain the CAT-like activity also contain a heat-labile inhibitor of (authentic) bacterial CAT.

Original languageEnglish
Pages (from-to)303-308
Number of pages6
JournalBBA - Gene Structure and Expression
Issue number3
StatePublished - 30 Nov 1990
Externally publishedYes


  • (Rat liver)
  • Bacteria
  • Chloramphenicol acetyltransferase
  • Thin layer chromatography
  • Transcription system
  • Transfection


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