TY - JOUR
T1 - Mapping of candidate tumor suppressor genes on chromosome 12 in adenoid cystic carcinoma
AU - Rutherford, Sue
AU - Hampton, Garret M.
AU - Frierson, Henry F.
AU - Moskaluk, Christopher A.
N1 - Funding Information:
We thank Sharon Birdsall and Mark Clem for assistance in histologic sectioning and Craig Rumpel for microsatellite LOH analysis and cell culture maintenance of the ACC3 cell line. We also thank Elizabeth Myers and Tyler Cooke for technical assistance. This work was supported by a grant from the National Institute of Dental and Craniofacial Research (RO1-DE-14694) and by a grant from the National Organization for Rare Disorders, Inc. (New Fairfield, CT, USA). The contents of this work are solely the responsibility of the authors and do not necessarily represent the official views of the National Institutes of Health.
PY - 2005
Y1 - 2005
N2 - Adenoid cystic carcinoma (ACC) is a common malignancy of salivary glands, for which the underlying genetic mechanisms of tumorigenesis are poorly understood. Prior studies in ACC have identified deletions in chromosome 12. To further characterize these changes, we performed an extensive LOH analysis in 58 ACC using a panel of 28 microsatellite markers. Results show 66% overall genetic loss. Three markers (D12S1713, D12S2196, D12S398) are contiguous and define a 6.84 Mb region of deletion at 12q13.11-q13.13. Two other markers (D12S2078, D12S1628) are also contiguous and define a 4.5 Mb region of deletion at 12q24.32-q24.33. The three remaining markers, D12S1056 at 12q14.1, D12S1051 at 12q23.1 and D12S1636 at 12q23.3 define smaller regions of deletion. An analysis of microarray gene expression profiling data available for ACC shows several genes with significant transcriptional downregulation that map to these areas of genetic deletion. This combined genetic and genomic analysis provides several candidate genes to test for functional tumor suppressor activity in ACC.
AB - Adenoid cystic carcinoma (ACC) is a common malignancy of salivary glands, for which the underlying genetic mechanisms of tumorigenesis are poorly understood. Prior studies in ACC have identified deletions in chromosome 12. To further characterize these changes, we performed an extensive LOH analysis in 58 ACC using a panel of 28 microsatellite markers. Results show 66% overall genetic loss. Three markers (D12S1713, D12S2196, D12S398) are contiguous and define a 6.84 Mb region of deletion at 12q13.11-q13.13. Two other markers (D12S2078, D12S1628) are also contiguous and define a 4.5 Mb region of deletion at 12q24.32-q24.33. The three remaining markers, D12S1056 at 12q14.1, D12S1051 at 12q23.1 and D12S1636 at 12q23.3 define smaller regions of deletion. An analysis of microarray gene expression profiling data available for ACC shows several genes with significant transcriptional downregulation that map to these areas of genetic deletion. This combined genetic and genomic analysis provides several candidate genes to test for functional tumor suppressor activity in ACC.
KW - Adenoid cystic carcinoma
KW - Genetic deletion
KW - Loss of heterozygosity
KW - Microarray
KW - Tumor suppressor gene
UR - http://www.scopus.com/inward/record.url?scp=27744499967&partnerID=8YFLogxK
U2 - 10.1038/labinvest.3700314
DO - 10.1038/labinvest.3700314
M3 - Article
C2 - 16025147
AN - SCOPUS:27744499967
SN - 0023-6837
VL - 85
SP - 1076
EP - 1085
JO - Laboratory Investigation
JF - Laboratory Investigation
IS - 9
ER -