Mass spectrometry-based biomarker discovery

Weidong Zhou*, Emanuel F. Petricoin, Caterina Longo

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

20 Scopus citations

Abstract

The discovery of candidate biomarkers within the entire proteome is one of the most important and challenging goals in proteomic research. Mass spectrometry-based proteomics is a modern and promising technology for semiquantitative and qualitative assessment of proteins, enabling protein sequencing and identification with exquisite accuracy and sensitivity. For mass spectrometry analysis, protein extractions from tissues or body fluids and subsequent protein fractionation represent an important and unavoidable step in the workflow for biomarker discovery. Following extraction of proteins, the protein mixture must be digested, reduced, alkylated, and cleaned up prior to mass spectrometry. The aim of our chapter is to provide comprehensible and practical lab procedures for sample digestion, protein fractionation, and subsequent mass spectrometry analysis.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages297-311
Number of pages15
DOIs
StatePublished - 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1606
ISSN (Print)1064-3745

Keywords

  • Biomarker discovery
  • In-gel digestion of proteins
  • In-solution digestion of proteins
  • LC-MS/MS
  • LTQ Orbitrap
  • Mass spectrometry
  • Proteomics
  • SEQUEST
  • Silver stain

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