Microsatellite primers for Australian and New Guinean pythons isolated with an efficient marker development method for related species

Philip W. Jordan; Amanda E. Goodman; Stephen Donnellan

doi:10.1046/j.1471-8286.2002.00135.x

Microsatellite primers for Australian and New Guinean pythons isolated with an efficient marker development method for related species

Philip W. Jordan, Amanda E. Goodman, Stephen Donnellan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Microsatellites are powerful molecular genetic markers for many evolutionary and biotechnological investigations, however, development of sufficient microsatellite markers is time-consuming and expensive especially considering the vast numbers of species for which they could be used. In light of the conservative nature of microsatellite loci between related species we describe an alternative approach to microsatellite development. A single round of microsatellite isolation enabled the characterization of sufficient loci for a large number of related python species. From 21 loci isolated in the focal species, an average of 86.2% were conserved within the other species while an average of 60.5% were polymorphic in all 13 python species analysed. Our approach will decrease significantly the expense and time required for microsatellite development for large numbers of related species.

Original language	English
Pages (from-to)	78-82
Number of pages	5
Journal	Molecular Ecology Notes
Volume	2
Issue number	1
DOIs	https://doi.org/10.1046/j.1471-8286.2002.00135.x
State	Published - 2002
Externally published	Yes

Keywords

DNA fingerprinting
Microsatellite
Python
Snake
Wildlife management

Access to Document

10.1046/j.1471-8286.2002.00135.x

Cite this

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title = "Microsatellite primers for Australian and New Guinean pythons isolated with an efficient marker development method for related species",

abstract = "Microsatellites are powerful molecular genetic markers for many evolutionary and biotechnological investigations, however, development of sufficient microsatellite markers is time-consuming and expensive especially considering the vast numbers of species for which they could be used. In light of the conservative nature of microsatellite loci between related species we describe an alternative approach to microsatellite development. A single round of microsatellite isolation enabled the characterization of sufficient loci for a large number of related python species. From 21 loci isolated in the focal species, an average of 86.2% were conserved within the other species while an average of 60.5% were polymorphic in all 13 python species analysed. Our approach will decrease significantly the expense and time required for microsatellite development for large numbers of related species.",

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AB - Microsatellites are powerful molecular genetic markers for many evolutionary and biotechnological investigations, however, development of sufficient microsatellite markers is time-consuming and expensive especially considering the vast numbers of species for which they could be used. In light of the conservative nature of microsatellite loci between related species we describe an alternative approach to microsatellite development. A single round of microsatellite isolation enabled the characterization of sufficient loci for a large number of related python species. From 21 loci isolated in the focal species, an average of 86.2% were conserved within the other species while an average of 60.5% were polymorphic in all 13 python species analysed. Our approach will decrease significantly the expense and time required for microsatellite development for large numbers of related species.

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