Abstract
Advances in methods for analyzing DNA, RNA, and proteins are transforming biomedical research and the practice of medicine. Traditional methods of assessing single genes or proteins are being complemented by advanced techniques that allow genomic or proteomic analysis of populations of cells or individual cells. The functions of genes and encoded proteins are determined in vivo using animal models that allow control of when and where genes are expressed. Molecular techniques enable gene-based therapies in which genes are corrected, supplemented, or suppressed for therapeutic benefit. The promise of precision medicine depends on our ability to implement these molecular techniques.
| Original language | English |
|---|---|
| Title of host publication | Dermatology |
| Subtitle of host publication | Volume 1-2, Fifth Edition |
| Publisher | Elsevier |
| Pages | 68-80 |
| Number of pages | 13 |
| Volume | 1 |
| ISBN (Electronic) | 9780702082252 |
| ISBN (Print) | 9780702084706 |
| DOIs | |
| State | Published - 1 Jan 2024 |
| Externally published | Yes |
Keywords
- CRISPR-cas9
- DNA sequencing
- ELISA
- enzyme-linked immunosorbent assay
- FISH
- flow cytometry
- fluorescence in situ hybridization
- gene-based therapy
- laser microdissection
- massively parallel sequencing
- next generation sequencing
- nucleic acid arrays
- PCR
- polymerase chain reaction
- proteome
- reverse transcription PCR
- RT-PCR
- transcriptome
- transgenic mice