Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana

Seth Offei Addo; Ronald Essah Bentil; Charlotte Adwoa Addae; Bernice Olivia Ama Baako; Kevin Nii Yartey; Jane Ansah‑Owusu; Christopher Nii Laryea Tawiah-Mensah; Bright Agbodzi; Selassie Kumordjie; Clara Yeboah; Victor Asoala; James C. Dunford; John Asiedu Larbi; Philip Kweku Baidoo; Michael David Wilson; Joseph W. Diclaro; Samuel K. Dadzie

doi:10.1007/s00705-024-05983-y

Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana

Seth Offei Addo^*, Ronald Essah Bentil, Charlotte Adwoa Addae, Bernice Olivia Ama Baako, Kevin Nii Yartey, Jane Ansah‑Owusu, Christopher Nii Laryea Tawiah-Mensah, Bright Agbodzi, Selassie Kumordjie, Clara Yeboah, Victor Asoala, James C. Dunford, John Asiedu Larbi, Philip Kweku Baidoo, Michael David Wilson, Joseph W. Diclaro, Samuel K. Dadzie^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Sampled ticks were screened for Crimean-Congo haemorrhagic fever virus (CCHFV) using an assay that targets the nucleoprotein gene region of the S segment, a conserved region of the CCHFV genome. Minimum infection rates of 0.34% and 0.10% were obtained when testing pools of Hyalomma rufipes and Amblyomma variegatum, respectively. Next-generation sequencing and phylogenetic analysis showed that the S and L segments of the CCHFV isolate clustered with those of similar isolates of genotype III. However, analysis of the M segment showed that reassortment had occurred, causing this segment to cluster with those of isolates of genotype I, providing the first evidence of such an occurrence in Ghana.

Original language	English
Article number	62
Journal	Archives of Virology
Volume	169
Issue number	3
DOIs	https://doi.org/10.1007/s00705-024-05983-y
State	Published - Mar 2024
Externally published	Yes

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Addo, S. O., Bentil, R. E., Addae, C. A., Baako, B. O. A., Yartey, K. N., Ansah‑Owusu, J., Tawiah-Mensah, C. N. L., Agbodzi, B., Kumordjie, S., Yeboah, C., Asoala, V., Dunford, J. C., Larbi, J. A., Baidoo, P. K., Wilson, M. D., Diclaro, J. W., & Dadzie, S. K. (2024). Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana. Archives of Virology, 169(3), Article 62. https://doi.org/10.1007/s00705-024-05983-y

@article{19d2513ed6bf4efa9c1a3380e28b2a67,

title = "Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana",

abstract = "Sampled ticks were screened for Crimean-Congo haemorrhagic fever virus (CCHFV) using an assay that targets the nucleoprotein gene region of the S segment, a conserved region of the CCHFV genome. Minimum infection rates of 0.34% and 0.10% were obtained when testing pools of Hyalomma rufipes and Amblyomma variegatum, respectively. Next-generation sequencing and phylogenetic analysis showed that the S and L segments of the CCHFV isolate clustered with those of similar isolates of genotype III. However, analysis of the M segment showed that reassortment had occurred, causing this segment to cluster with those of isolates of genotype I, providing the first evidence of such an occurrence in Ghana.",

author = "Addo, {Seth Offei} and Bentil, {Ronald Essah} and Addae, {Charlotte Adwoa} and Baako, {Bernice Olivia Ama} and Yartey, {Kevin Nii} and Jane Ansah‑Owusu and Tawiah-Mensah, {Christopher Nii Laryea} and Bright Agbodzi and Selassie Kumordjie and Clara Yeboah and Victor Asoala and Dunford, {James C.} and Larbi, {John Asiedu} and Baidoo, {Philip Kweku} and Wilson, {Michael David} and Diclaro, {Joseph W.} and Dadzie, {Samuel K.}",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024.",

year = "2024",

month = mar,

doi = "10.1007/s00705-024-05983-y",

language = "English",

volume = "169",

journal = "Archives of Virology",

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Addo, SO, Bentil, RE, Addae, CA, Baako, BOA, Yartey, KN, Ansah‑Owusu, J, Tawiah-Mensah, CNL, Agbodzi, B, Kumordjie, S, Yeboah, C, Asoala, V, Dunford, JC, Larbi, JA, Baidoo, PK, Wilson, MD, Diclaro, JW & Dadzie, SK 2024, 'Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana', Archives of Virology, vol. 169, no. 3, 62. https://doi.org/10.1007/s00705-024-05983-y

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T1 - Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana

AU - Addo, Seth Offei

AU - Bentil, Ronald Essah

AU - Addae, Charlotte Adwoa

AU - Baako, Bernice Olivia Ama

AU - Yartey, Kevin Nii

AU - Ansah‑Owusu, Jane

AU - Tawiah-Mensah, Christopher Nii Laryea

AU - Agbodzi, Bright

AU - Kumordjie, Selassie

AU - Yeboah, Clara

AU - Asoala, Victor

AU - Dunford, James C.

AU - Larbi, John Asiedu

AU - Baidoo, Philip Kweku

AU - Wilson, Michael David

AU - Diclaro, Joseph W.

AU - Dadzie, Samuel K.

N1 - Publisher Copyright: © The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2024.

PY - 2024/3

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AB - Sampled ticks were screened for Crimean-Congo haemorrhagic fever virus (CCHFV) using an assay that targets the nucleoprotein gene region of the S segment, a conserved region of the CCHFV genome. Minimum infection rates of 0.34% and 0.10% were obtained when testing pools of Hyalomma rufipes and Amblyomma variegatum, respectively. Next-generation sequencing and phylogenetic analysis showed that the S and L segments of the CCHFV isolate clustered with those of similar isolates of genotype III. However, analysis of the M segment showed that reassortment had occurred, causing this segment to cluster with those of isolates of genotype I, providing the first evidence of such an occurrence in Ghana.

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ER -

Molecular identification of Crimean-Congo haemorrhagic fever virus in Hyalomma rufipes and Amblyomma variegatum in the Upper East Region of Ghana

Abstract

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