Monitoring proteins and protein networks using reverse phase protein arrays

Bridget Wilson, Lance A. Liotta, Emanuel Petricoin*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

33 Scopus citations


Recent advances in high throughput, high content "omic" technologies coupled with clinical information has lead to the expectation that the complexity of the molecular information generated will lead to more robust scientific research as well as the expectation that overarching therapeutic approaches will be patient-tailored to the underlying specific molecular defects of the disease. As disease understanding progresses and more therapeutics, which predominately target proteins, are developed there is a need to more confidently determine the protein signaling events that can be correlated with drug response since the deranged protein signaling networks are often the drug target itself. In this environment, the Reverse Phase Protein Microarray (RPMA) can be utilized to address the needs of both clinical screening and disease understanding through its ability to provide an unmatched functional and highly multiplexed signaling network level mapping of ongoing signaling activation, coupled with the ability of the platform to provide this information reproducibly from a tiny needle biopsy specimen or fine needle aspirate. This platform has now been utilized for biomarker discovery/validation and advancements in disease understanding both in the clinic and at the bench in the fields of cancer, liver disease, immunological disorders, and bacterial infection.

Original languageEnglish
Pages (from-to)225-232
Number of pages8
JournalDisease Markers
Issue number4
StatePublished - 2010
Externally publishedYes


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