TY - JOUR
T1 - MS analysis reveals O-methylation of L-lactate dehydrogenase from pancreatic ductal adenocarcinoma cells
AU - Zhou, Weidong
AU - Capello, Michela
AU - Fredolini, Claudia
AU - Racanicchi, Leda
AU - Piemonti, Lorenzo
AU - Liotta, Lance A.
AU - Novelli, Francesco
AU - Petricoin, Emanuel F.
PY - 2012/7
Y1 - 2012/7
N2 - L-lactate dehydrogenase (LDH) converts pyruvate to lactate when oxygen is absent or in short supply, and the enzyme plays a crucial role in cancer metabolism. The functions of many mammalian proteins are modulated by posttranslational modifications (PTMs), and it has been reported that LDH was subjected to several PTMs, including phosphorylation, acetylation, and methylation. In this present work, we characterized the PTMs of LDH from pancreatic ductal adenocarcinoma (PDAC) cells by electrophoresis and mass spectrometry, and identified 13 O-methylated residues from the enzyme. In addition, our qualitative analysis revealed differential methylation of LDH from normal duct cells. The preliminary findings from this study provide important biochemical information toward further understanding of the LDH modifications and their functional significance in pathophysiological processes of pancreatic cancer.
AB - L-lactate dehydrogenase (LDH) converts pyruvate to lactate when oxygen is absent or in short supply, and the enzyme plays a crucial role in cancer metabolism. The functions of many mammalian proteins are modulated by posttranslational modifications (PTMs), and it has been reported that LDH was subjected to several PTMs, including phosphorylation, acetylation, and methylation. In this present work, we characterized the PTMs of LDH from pancreatic ductal adenocarcinoma (PDAC) cells by electrophoresis and mass spectrometry, and identified 13 O-methylated residues from the enzyme. In addition, our qualitative analysis revealed differential methylation of LDH from normal duct cells. The preliminary findings from this study provide important biochemical information toward further understanding of the LDH modifications and their functional significance in pathophysiological processes of pancreatic cancer.
KW - L-lactate dehydrogenase
KW - Mass spectrometry
KW - Metabolism
KW - Methylation
KW - Pancreatic ductal adenocarcinoma
UR - http://www.scopus.com/inward/record.url?scp=84862979557&partnerID=8YFLogxK
U2 - 10.1002/elps.201200017
DO - 10.1002/elps.201200017
M3 - Article
C2 - 22740473
AN - SCOPUS:84862979557
SN - 0173-0835
VL - 33
SP - 1850
EP - 1854
JO - Electrophoresis
JF - Electrophoresis
IS - 12
ER -