Multiphoton tissue FRET demonstrates in vivo BAD/Bcl-xL heterodimerization in injured axons following traumatic brain injury in rats

James D. Mills; James R. Stone; David O. Okonkwo; Ammasi Periasamy; Gregory A. Helm

doi:10.1117/12.533830

Multiphoton tissue FRET demonstrates in vivo BAD/Bcl-xL heterodimerization in injured axons following traumatic brain injury in rats

James D. Mills, James R. Stone^*, David O. Okonkwo, Ammasi Periasamy, Gregory A. Helm

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

Abstract

A modified method of multiphoton fluorescence resonance energy transfer (FRET) for investigation of traumatic brain injury (TBI) is discussed. The method allows for the direct assessment of protein-protein interactions in tissue sections through the use of a dual-label immunofluorescent approach. It was found that the bcl-2-related proteins BAD and Bcl-xL heterodimerize in a pro-apoptotic fashion within traumatically injured axons following TBI. The results further define the activation of a calcineurin mediated, BAD/Bcl-xL apoptic pathway as a secondary mechanism of injury in TBI.

Original language	English
Pages (from-to)	52-62
Number of pages	11
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5323
DOIs	https://doi.org/10.1117/12.533830
State	Published - 2004
Externally published	Yes
Event	Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV - San Jose, CA, United States Duration: 25 Jan 2004 → 27 Jan 2004

Keywords

BAD
Bcl-xL
Caspase 3
Cytochrome c
Fluorescent Resonance Energy Transfer (FRET)
Traumatic axonal injury

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10.1117/12.533830

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title = "Multiphoton tissue FRET demonstrates in vivo BAD/Bcl-xL heterodimerization in injured axons following traumatic brain injury in rats",

abstract = "A modified method of multiphoton fluorescence resonance energy transfer (FRET) for investigation of traumatic brain injury (TBI) is discussed. The method allows for the direct assessment of protein-protein interactions in tissue sections through the use of a dual-label immunofluorescent approach. It was found that the bcl-2-related proteins BAD and Bcl-xL heterodimerize in a pro-apoptotic fashion within traumatically injured axons following TBI. The results further define the activation of a calcineurin mediated, BAD/Bcl-xL apoptic pathway as a secondary mechanism of injury in TBI.",

keywords = "BAD, Bcl-xL, Caspase 3, Cytochrome c, Fluorescent Resonance Energy Transfer (FRET), Traumatic axonal injury",

author = "Mills, {James D.} and Stone, {James R.} and Okonkwo, {David O.} and Ammasi Periasamy and Helm, {Gregory A.}",

year = "2004",

doi = "10.1117/12.533830",

language = "English",

volume = "5323",

pages = "52--62",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

note = "Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV ; Conference date: 25-01-2004 Through 27-01-2004",

}

Multiphoton tissue FRET demonstrates in vivo BAD/Bcl-xL heterodimerization in injured axons following traumatic brain injury in rats. / Mills, James D.; Stone, James R.; Okonkwo, David O. et al.
In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 5323, 2004, p. 52-62.

Research output: Contribution to journal › Conference article › peer-review

TY - JOUR

T1 - Multiphoton tissue FRET demonstrates in vivo BAD/Bcl-xL heterodimerization in injured axons following traumatic brain injury in rats

AU - Mills, James D.

AU - Stone, James R.

AU - Okonkwo, David O.

AU - Periasamy, Ammasi

AU - Helm, Gregory A.

PY - 2004

Y1 - 2004

N2 - A modified method of multiphoton fluorescence resonance energy transfer (FRET) for investigation of traumatic brain injury (TBI) is discussed. The method allows for the direct assessment of protein-protein interactions in tissue sections through the use of a dual-label immunofluorescent approach. It was found that the bcl-2-related proteins BAD and Bcl-xL heterodimerize in a pro-apoptotic fashion within traumatically injured axons following TBI. The results further define the activation of a calcineurin mediated, BAD/Bcl-xL apoptic pathway as a secondary mechanism of injury in TBI.

AB - A modified method of multiphoton fluorescence resonance energy transfer (FRET) for investigation of traumatic brain injury (TBI) is discussed. The method allows for the direct assessment of protein-protein interactions in tissue sections through the use of a dual-label immunofluorescent approach. It was found that the bcl-2-related proteins BAD and Bcl-xL heterodimerize in a pro-apoptotic fashion within traumatically injured axons following TBI. The results further define the activation of a calcineurin mediated, BAD/Bcl-xL apoptic pathway as a secondary mechanism of injury in TBI.

KW - BAD

KW - Bcl-xL

KW - Caspase 3

KW - Cytochrome c

KW - Fluorescent Resonance Energy Transfer (FRET)

KW - Traumatic axonal injury

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U2 - 10.1117/12.533830

DO - 10.1117/12.533830

M3 - Conference article

AN - SCOPUS:5644266228

SN - 0277-786X

VL - 5323

SP - 52

EP - 62

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Progress in Biomedical Optics and Imaging - Multiphoton Microscopy in the Biomedical Sciences IV

Y2 - 25 January 2004 through 27 January 2004

ER -

Multiphoton tissue FRET demonstrates in vivo BAD/Bcl-xL heterodimerization in injured axons following traumatic brain injury in rats

Abstract

Keywords

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