Multiplexed cell signaling analysis of human breast cancer applications for personalized therapy

Julia D. Wulfkuhle; Runa Speer; Mariaelena Pierobon; Julie Laird; Virginia Espina; Jianghong Deng; Enzo Mammano; Herry X. Yang; Sandra M. Swain; Donato Nitti; Laura J. Esserman; Claudio Belluco; Lance A. Liotta; Emanuel F. Petricoin

doi:10.1021/pr7008127

Multiplexed cell signaling analysis of human breast cancer applications for personalized therapy

Julia D. Wulfkuhle, Runa Speer, Mariaelena Pierobon, Julie Laird, Virginia Espina, Jianghong Deng, Enzo Mammano, Herry X. Yang, Sandra M. Swain, Donato Nitti, Laura J. Esserman, Claudio Belluco, Lance A. Liotta, Emanuel F. Petricoin

Research output: Contribution to journal › Article › peer-review

124 Scopus citations

Abstract

Phosphoprotein driven cellular signaling events represent most of the new molecular targets for cancer treatment. Application of reverse-phase protein microarray technology for the study of ongoing signaling activity within breast tumor specimens holds great potential for elucidating and profiling signaling activity in real-time for patient-tailored therapy. Analysis of laser capture microdissection primary human breast tumors and metastatic lesions reveals pathway specific profiles and a new way to classify cancer based on functional signaling portraits. Moreover, the data demonstrate the requirement of laser capture microdissection for analysis and reveal the metastasis-specific changes that occur within a new microenvironment. Analysis of biopsy material from clinical trials for targeted therapeutics demonstrates the feasibility and utility of comprehensive signal pathway activation profiling for molecular analysis.

Original language	English
Pages (from-to)	1508-1517
Number of pages	10
Journal	Journal of Proteome Research
Volume	7
Issue number	4
DOIs	https://doi.org/10.1021/pr7008127
State	Published - Apr 2008
Externally published	Yes

Keywords

Breast cancer
Cell signaling
Phosphoproteomics
Protein microarray
Proteomics
Tailored therapy

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10.1021/pr7008127

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Wulfkuhle, J. D., Speer, R., Pierobon, M., Laird, J., Espina, V., Deng, J., Mammano, E., Yang, H. X., Swain, S. M., Nitti, D., Esserman, L. J., Belluco, C., Liotta, L. A., & Petricoin, E. F. (2008). Multiplexed cell signaling analysis of human breast cancer applications for personalized therapy. Journal of Proteome Research, 7(4), 1508-1517. https://doi.org/10.1021/pr7008127

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title = "Multiplexed cell signaling analysis of human breast cancer applications for personalized therapy",

abstract = "Phosphoprotein driven cellular signaling events represent most of the new molecular targets for cancer treatment. Application of reverse-phase protein microarray technology for the study of ongoing signaling activity within breast tumor specimens holds great potential for elucidating and profiling signaling activity in real-time for patient-tailored therapy. Analysis of laser capture microdissection primary human breast tumors and metastatic lesions reveals pathway specific profiles and a new way to classify cancer based on functional signaling portraits. Moreover, the data demonstrate the requirement of laser capture microdissection for analysis and reveal the metastasis-specific changes that occur within a new microenvironment. Analysis of biopsy material from clinical trials for targeted therapeutics demonstrates the feasibility and utility of comprehensive signal pathway activation profiling for molecular analysis.",

keywords = "Breast cancer, Cell signaling, Phosphoproteomics, Protein microarray, Proteomics, Tailored therapy",

author = "Wulfkuhle, {Julia D.} and Runa Speer and Mariaelena Pierobon and Julie Laird and Virginia Espina and Jianghong Deng and Enzo Mammano and Yang, {Herry X.} and Swain, {Sandra M.} and Donato Nitti and Esserman, {Laura J.} and Claudio Belluco and Liotta, {Lance A.} and Petricoin, {Emanuel F.}",

year = "2008",

month = apr,

doi = "10.1021/pr7008127",

language = "English",

volume = "7",

pages = "1508--1517",

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AU - Wulfkuhle, Julia D.

AU - Speer, Runa

AU - Pierobon, Mariaelena

AU - Laird, Julie

AU - Espina, Virginia

AU - Deng, Jianghong

AU - Mammano, Enzo

AU - Yang, Herry X.

AU - Swain, Sandra M.

AU - Nitti, Donato

AU - Esserman, Laura J.

AU - Belluco, Claudio

AU - Liotta, Lance A.

AU - Petricoin, Emanuel F.

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N2 - Phosphoprotein driven cellular signaling events represent most of the new molecular targets for cancer treatment. Application of reverse-phase protein microarray technology for the study of ongoing signaling activity within breast tumor specimens holds great potential for elucidating and profiling signaling activity in real-time for patient-tailored therapy. Analysis of laser capture microdissection primary human breast tumors and metastatic lesions reveals pathway specific profiles and a new way to classify cancer based on functional signaling portraits. Moreover, the data demonstrate the requirement of laser capture microdissection for analysis and reveal the metastasis-specific changes that occur within a new microenvironment. Analysis of biopsy material from clinical trials for targeted therapeutics demonstrates the feasibility and utility of comprehensive signal pathway activation profiling for molecular analysis.

AB - Phosphoprotein driven cellular signaling events represent most of the new molecular targets for cancer treatment. Application of reverse-phase protein microarray technology for the study of ongoing signaling activity within breast tumor specimens holds great potential for elucidating and profiling signaling activity in real-time for patient-tailored therapy. Analysis of laser capture microdissection primary human breast tumors and metastatic lesions reveals pathway specific profiles and a new way to classify cancer based on functional signaling portraits. Moreover, the data demonstrate the requirement of laser capture microdissection for analysis and reveal the metastasis-specific changes that occur within a new microenvironment. Analysis of biopsy material from clinical trials for targeted therapeutics demonstrates the feasibility and utility of comprehensive signal pathway activation profiling for molecular analysis.

KW - Breast cancer

KW - Cell signaling

KW - Phosphoproteomics

KW - Protein microarray

KW - Proteomics

KW - Tailored therapy

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JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 4

ER -

Multiplexed cell signaling analysis of human breast cancer applications for personalized therapy

Abstract

Keywords

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