N-Acetylaspartylglutamate identified in the rat retinal ganglion cells and their projections in the brain

Kevin J. Anderson; Mark A. Borja; Carl W. Cotman; John R. Moffett; M. A.A. Namboodiri; Joseph H. Neale

doi:10.1016/0006-8993(87)90696-2

N-Acetylaspartylglutamate identified in the rat retinal ganglion cells and their projections in the brain

Kevin J. Anderson^*, Mark A. Borja, Carl W. Cotman, John R. Moffett, M. A.A. Namboodiri, Joseph H. Neale

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

77 Scopus citations

Abstract

N-Acetylaspartyglutamate-like immunoreactivity (NAAG-L) was identified in retinal ganglion cell bodies and their axons. The presence of the dipeptide in ganglion cell projection areas, the lateral geniculate nucleus (LGN) and superior colliculus (SC), was confirmed following NAAG purification from these tissues by a high-performance liquid chromatographic method. NAAG-L was identified in the optic tract as well as within fibers and puncta in the LGN and SC. The hypothesis that NAAG is present within ganglion cell axons in the brain was tested by unilateral enucleation which resulted in loss of NAAG and NAAG-L within the contralateral LGN and SC.

Original language	English
Pages (from-to)	172-177
Number of pages	6
Journal	Brain Research
Volume	411
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(87)90696-2
State	Published - 12 May 1987

Keywords

Dipeptide
High-performance liquid chromatography
Immunohistochemistry
Lateral geniculate nucleus
Optic tract
Retina
Superior colliculus

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10.1016/0006-8993(87)90696-2

Cite this

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title = "N-Acetylaspartylglutamate identified in the rat retinal ganglion cells and their projections in the brain",

abstract = "N-Acetylaspartyglutamate-like immunoreactivity (NAAG-L) was identified in retinal ganglion cell bodies and their axons. The presence of the dipeptide in ganglion cell projection areas, the lateral geniculate nucleus (LGN) and superior colliculus (SC), was confirmed following NAAG purification from these tissues by a high-performance liquid chromatographic method. NAAG-L was identified in the optic tract as well as within fibers and puncta in the LGN and SC. The hypothesis that NAAG is present within ganglion cell axons in the brain was tested by unilateral enucleation which resulted in loss of NAAG and NAAG-L within the contralateral LGN and SC.",

keywords = "Dipeptide, High-performance liquid chromatography, Immunohistochemistry, Lateral geniculate nucleus, Optic tract, Retina, Superior colliculus",

author = "Anderson, {Kevin J.} and Borja, {Mark A.} and Cotman, {Carl W.} and Moffett, {John R.} and Namboodiri, {M. A.A.} and Neale, {Joseph H.}",

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T1 - N-Acetylaspartylglutamate identified in the rat retinal ganglion cells and their projections in the brain

AU - Anderson, Kevin J.

AU - Borja, Mark A.

AU - Cotman, Carl W.

AU - Moffett, John R.

AU - Namboodiri, M. A.A.

AU - Neale, Joseph H.

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AB - N-Acetylaspartyglutamate-like immunoreactivity (NAAG-L) was identified in retinal ganglion cell bodies and their axons. The presence of the dipeptide in ganglion cell projection areas, the lateral geniculate nucleus (LGN) and superior colliculus (SC), was confirmed following NAAG purification from these tissues by a high-performance liquid chromatographic method. NAAG-L was identified in the optic tract as well as within fibers and puncta in the LGN and SC. The hypothesis that NAAG is present within ganglion cell axons in the brain was tested by unilateral enucleation which resulted in loss of NAAG and NAAG-L within the contralateral LGN and SC.

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KW - High-performance liquid chromatography

KW - Immunohistochemistry

KW - Lateral geniculate nucleus

KW - Optic tract

KW - Retina

KW - Superior colliculus

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