Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells

Rajib Sengupta, Timothy R. Billiar, James L. Atkins, Valerian E. Kagan, Detcho A. Stoyanovsky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-l-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted in endogenous denitrosation and reactivation of CASP-SH. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, LA markedly reactivated CASP-SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S-denitrosation.

Original languageEnglish
Pages (from-to)3525-3530
Number of pages6
JournalFEBS Letters
Volume583
Issue number21
DOIs
StatePublished - 3 Nov 2009
Externally publishedYes

Keywords

  • Caspase
  • Denitrosation
  • Dihydrolipoic
  • Lipoic acid
  • Nitric oxide

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