Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells

Rajib Sengupta; Timothy R. Billiar; James L. Atkins; Valerian E. Kagan; Detcho A. Stoyanovsky

doi:10.1016/j.febslet.2009.10.016

Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells

Rajib Sengupta, Timothy R. Billiar, James L. Atkins, Valerian E. Kagan, Detcho A. Stoyanovsky^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-l-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted in endogenous denitrosation and reactivation of CASP-SH. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, LA markedly reactivated CASP-SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S-denitrosation.

Original language	English
Pages (from-to)	3525-3530
Number of pages	6
Journal	FEBS Letters
Volume	583
Issue number	21
DOIs	https://doi.org/10.1016/j.febslet.2009.10.016
State	Published - 3 Nov 2009
Externally published	Yes

Keywords

Caspase
Denitrosation
Dihydrolipoic
Lipoic acid
Nitric oxide

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10.1016/j.febslet.2009.10.016

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title = "Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells",

abstract = "Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-l-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted in endogenous denitrosation and reactivation of CASP-SH. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, LA markedly reactivated CASP-SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S-denitrosation.",

keywords = "Caspase, Denitrosation, Dihydrolipoic, Lipoic acid, Nitric oxide",

author = "Rajib Sengupta and Billiar, {Timothy R.} and Atkins, {James L.} and Kagan, {Valerian E.} and Stoyanovsky, {Detcho A.}",

note = "Funding Information: This work is supported by NIH grant GM044100 and Walter Reed Army Institute of Research grants W81XWH-09-P-0631 and W81XWH-06-1-0247 . Funding Information: Disclaimer: The views, opinions, and/or findings contained herein are those of the authors and should not be construed as an official position, policy, or decision of the Department of the Army, the Department of the Navy or the Department of Defense. This work was funded in part by a grant from the Office of Naval Research #42237.",

year = "2009",

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doi = "10.1016/j.febslet.2009.10.016",

language = "English",

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T1 - Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells

AU - Sengupta, Rajib

AU - Billiar, Timothy R.

AU - Atkins, James L.

AU - Kagan, Valerian E.

AU - Stoyanovsky, Detcho A.

N1 - Funding Information: This work is supported by NIH grant GM044100 and Walter Reed Army Institute of Research grants W81XWH-09-P-0631 and W81XWH-06-1-0247 . Funding Information: Disclaimer: The views, opinions, and/or findings contained herein are those of the authors and should not be construed as an official position, policy, or decision of the Department of the Army, the Department of the Navy or the Department of Defense. This work was funded in part by a grant from the Office of Naval Research #42237.

PY - 2009/11/3

Y1 - 2009/11/3

N2 - Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-l-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted in endogenous denitrosation and reactivation of CASP-SH. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, LA markedly reactivated CASP-SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S-denitrosation.

AB - Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S-nitrosocaspase 3 (CASP-SNO). In HepG2 cells, S-nitroso-l-cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP-SH), while a subsequent incubation of the cells in SNCEE-free medium resulted in endogenous denitrosation and reactivation of CASP-SH. The latter process was inhibited in thioredoxin reductase-deficient HepG2 cells, in which, however, LA markedly reactivated CASP-SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S-denitrosation.

KW - Caspase

KW - Denitrosation

KW - Dihydrolipoic

KW - Lipoic acid

KW - Nitric oxide

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DO - 10.1016/j.febslet.2009.10.016

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SN - 0014-5793

VL - 583

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EP - 3530

JO - FEBS Letters

JF - FEBS Letters

IS - 21

ER -

Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells

Abstract

Keywords

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