NK cell degranulation as a marker for measuring antibody-dependent cytotoxicity in neutralizing and non-neutralizing human sera from dengue patients

Peifang Sun*, Brian J. Morrison, Charmagne G. Beckett, Zhaodong Liang, Nishith Nagabhushana, An Li, Kevin R. Porter, Maya Williams

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

The study assessed antibody-dependent NK cell degranulation, a biomarker relevant to antibody-dependent cell cytotoxicity (ADCC), to analyze dengue immune sera. We first determined binding intensity of patient sera to the surface of DENV-infected cells and examined the types of antigens expressed on infected cells. Antigens from pre-membrane (PreM) and envelope (E), but not from NS proteins were detected on the surface of infected cells. After adding NK cells to infected target cells previously treated with patient sera, rapid NK cell degranulation was observed. Non-neutralizing patient sera generated comparable NK cell degranulation as that of neutralizing sera, suggesting ADCC may be a protective mechanism apart from Ab neutralization. The level of NK cell degranulation varied dramatically among human individuals and was associated with the level of CD16 expression on NK cells, informing on the complexity of ADCC among human population.

Original languageEnglish
Pages (from-to)24-30
Number of pages7
JournalJournal of Immunological Methods
Volume441
DOIs
StatePublished - 1 Feb 2017
Externally publishedYes

Keywords

  • ADCC
  • CD16
  • Dengue serum
  • Dengue virus
  • NK cell degranulation

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