Novel technologies in studying chronic liver disease

Ancha Baranova, Emanuel Petricoin, Lance Liotta, Zobair M. Younossi

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review


BACKGROUND A.“high-throughput revolution,” unfolding in modern clinical science, has led to a significant increase in knowledge describing genome, transcriptome, and proteome in complex human diseases, including chronic liver diseases. Genome-based methods of the assessment of the cellular functioning highlight the differences between individuals known as Single Nucleotide Polymorphisms (SNPs). SNPs are a DNA.sequence variations of a single nucleotide - A. T, C, or G - that are commonly present in a healthy human population. For example, two sequenced DNA.fragments from different individuals, A.TCCCTA.and A.TGCCTA. contain a difference in a single nucleotide. In this case, we usually say that there are two SNP alleles: C and G. SNPs may fall within coding sequences of genes or their noncoding, regulatory regions. SNPs that are not in protein coding regions may still have consequences for the alternative splicing of the mRNA. for the transcription factor binding to the promoter, or to the annealing of the gene-regulating noncoding RNA. Of Ten, noncoding SNPs lead to the alterations in the cellular levels of the mRNA.encoded for the particular protein, and, consequently, to the changes in the protein concentrations. A. the concentrations of the proteins differ between individuals, humans differ in their degree of predisposition to various chronic diseases, including chronic liver diseases (CLD). Transcriptomics and proteomics methods of cellular function assessment aim at the collection of the molecular “snapshots” reflecting relative levels of the mRNA. (transcriptome) or proteins (proteome) in the particular human tissues.

Original languageEnglish
Title of host publicationPractical Management of Liver Diseases
PublisherCambridge University Press
Number of pages21
ISBN (Electronic)9780511547416
ISBN (Print)9780521684897
StatePublished - 1 Jan 2008
Externally publishedYes


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