Nucleic acid (DNA) immunization as a platform for dengue vaccine development

Kevin R. Porter*, Kanakatte Raviprakash

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Since the early 1990s, DNA immunization has been used as a platform for developing a tetravalent dengue vaccine in response to the high priority need for protecting military personnel deployed to dengue endemic regions of the world. Several approaches have been explored ranging from naked DNA immunization to the use of live virus vectors to deliver the targeted genes for expression. Pre-clinical animal studies were largely successful in generating anti-dengue cellular and humoral immune responses that were protective either completely or partially against challenge with live dengue virus. However, Phase 1 clinical evaluation of a prototype monovalent dengue 1 DNA vaccine expressing prM and E genes revealed anti-dengue T cell IFNγ responses, but poor neutralizing antibody responses. These less than optimal results are thought to be due to poor uptake and expression of the DNA vaccine plasmids. Because DNA immunization as a vaccine platform has the advantages of ease of manufacture, flexible genetic manipulation and enhanced stability, efforts continue to improve the immunogenicity of these vaccines using a variety of methods.

Original languageEnglish
Pages (from-to)7135-7140
Number of pages6
JournalVaccine
Volume33
Issue number50
DOIs
StatePublished - 10 Dec 2015
Externally publishedYes

Keywords

  • Biojector
  • Clinical trials
  • DNA vaccines
  • Dengue
  • Non-human primates
  • Plasmid
  • Shuffled DNA vaccine

Fingerprint

Dive into the research topics of 'Nucleic acid (DNA) immunization as a platform for dengue vaccine development'. Together they form a unique fingerprint.

Cite this