Nucleic acid scavenging microfiber mesh inhibits trauma-induced inflammation and thrombosis

Jaewoo Lee*, Jennifer G. Jackman, Jean Kwun, Miriam Manook, Angelo Moreno, Eric A. Elster, Allan D. Kirk, Kam W. Leong, Bruce A. Sullenger

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Trauma patients produce a host of danger signals and high levels of damage-associated molecular patterns (DAMPs) after cellular injury and tissue damage. These DAMPs are directly and indirectly involved in the pathogenesis of various inflammatory and thrombotic complications in patients with severe injuries. No effective therapeutic agents for the removal of DAMPs from blood or tissue fluid have been developed. Herein, we demonstrated that nucleic acid binding polymers, e.g., polyethylenimine (PEI) and polyamidoamine dendrimers, immobilized onto electrospun microfiber mesh can effectively capture various DAMPs, such as extracellular DNAs and high mobility group box 1 (HMGB1). Furthermore, treatment with PEI-immobilized microfiber mesh abrogated the ability of DAMPs, released from dead and dying cells in culture or found in patients following traumatic injury, to activate innate immune responses and coagulation in vitro and in vivo. Nucleic acid scavenging microfiber meshes represent an effective strategy to combat inflammation and thrombosis in trauma.

Original languageEnglish
Pages (from-to)94-102
Number of pages9
JournalBiomaterials
Volume120
DOIs
StatePublished - 1 Mar 2017
Externally publishedYes

Keywords

  • Inflammation
  • Microfiber mesh
  • Nucleic acid scavenger
  • Thrombosis
  • Toll like receptor

Fingerprint

Dive into the research topics of 'Nucleic acid scavenging microfiber mesh inhibits trauma-induced inflammation and thrombosis'. Together they form a unique fingerprint.

Cite this