Oligonucleotide probe for detection of the enteropathogenic Escherichia coli (EPEC) adherence factor of localized adherent EPEC

A. E. Jerse, W. C. Martin, J. E. Galen, J. B. Kaper*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The enteropathogenic Escherichia coli adherence factor (EAF) probe detects isolates of enteropathogenic E. coli that exhibit localized adherence to HEp-2 cells. A 21-base oligonucleotide probe was constructed on the basis of a sequence from within the 1-kb EAF probe and was shown to have greater sensitivity and specificity than the EAF fragment probe in detecting localized adherent E. coli.

Original languageEnglish
Pages (from-to)2842-2844
Number of pages3
JournalJournal of Clinical Microbiology
Volume28
Issue number12
DOIs
StatePublished - 1990
Externally publishedYes

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