Oligonucleotide probe for detection of the enteropathogenic Escherichia coli (EPEC) adherence factor of localized adherent EPEC

A. E. Jerse; W. C. Martin; J. E. Galen; J. B. Kaper

doi:10.1128/jcm.28.12.2842-2844.1990

Oligonucleotide probe for detection of the enteropathogenic Escherichia coli (EPEC) adherence factor of localized adherent EPEC

A. E. Jerse, W. C. Martin, J. E. Galen, J. B. Kaper^*

^*Corresponding author for this work

Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

The enteropathogenic Escherichia coli adherence factor (EAF) probe detects isolates of enteropathogenic E. coli that exhibit localized adherence to HEp-2 cells. A 21-base oligonucleotide probe was constructed on the basis of a sequence from within the 1-kb EAF probe and was shown to have greater sensitivity and specificity than the EAF fragment probe in detecting localized adherent E. coli.

Original language	English
Pages (from-to)	2842-2844
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	28
Issue number	12
DOIs	https://doi.org/10.1128/jcm.28.12.2842-2844.1990
State	Published - 1990

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10.1128/jcm.28.12.2842-2844.1990

Cite this

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title = "Oligonucleotide probe for detection of the enteropathogenic Escherichia coli (EPEC) adherence factor of localized adherent EPEC",

abstract = "The enteropathogenic Escherichia coli adherence factor (EAF) probe detects isolates of enteropathogenic E. coli that exhibit localized adherence to HEp-2 cells. A 21-base oligonucleotide probe was constructed on the basis of a sequence from within the 1-kb EAF probe and was shown to have greater sensitivity and specificity than the EAF fragment probe in detecting localized adherent E. coli.",

author = "Jerse, {A. E.} and Martin, {W. C.} and Galen, {J. E.} and Kaper, {J. B.}",

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AU - Galen, J. E.

AU - Kaper, J. B.

PY - 1990

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AB - The enteropathogenic Escherichia coli adherence factor (EAF) probe detects isolates of enteropathogenic E. coli that exhibit localized adherence to HEp-2 cells. A 21-base oligonucleotide probe was constructed on the basis of a sequence from within the 1-kb EAF probe and was shown to have greater sensitivity and specificity than the EAF fragment probe in detecting localized adherent E. coli.

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DO - 10.1128/jcm.28.12.2842-2844.1990

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