TY - JOUR
T1 - Optimal dose and schedule of an HER-2/neu (E75) peptide vaccine to prevent breast cancer recurrence
T2 - From US Military Cancer Institute Clinical Trials Group Study I-01 and I-02
AU - Holmes, Jarrod P.
AU - Gates, Jeremy D.
AU - Benavides, Linda C.
AU - Hueman, Matthew T.
AU - Carmichael, Mark G.
AU - Patil, Ritesh
AU - Craig, Dianna
AU - Mittendorf, Elizabeth A.
AU - Stojadinovic, Alexander
AU - Ponniah, Sathibalan
AU - Peoples, George E.
PY - 2008/10/1
Y1 - 2008/10/1
N2 - BACKGROUND. E75, a HER-2/neu-derived peptide, was administered as a preventive vaccine with granulocyte-macrophage-colony-stimulating factor (GM-CSF) in disease-free lymph node-positive (NP) and lymph node-negative (NN) breast cancer (BCa) patients. The optimal biologic dose (OBD) was determined based on toxicity and immunologic response. METHODS. Patients were vaccinated over 6 months (3, 4, or 6 times) with different doses of E75 plus GM-CSF. Toxicities were graded per National Cancer Institute Common Terminology Criteria. GM-CSF was reduced for significant toxicity. Immunologic response was measured by delayed type hypersensitivity test (DTH), and E75-specific CD8 + T-cells were quantified with human leukocyte antigen-A2: immunoglobulin G dimer and flow cytometry. RESULTS. Ninety-nine patients (48 NP and 51 NN) were vaccinated in 7 dose groups. The OBD was 1000 lg E75 plus 250 μg GM-CSF monthly x 6. The optimal dose group (ODG, n = 29) experienced similar toxicities to the suboptimal dose group (SDG, n = 70), which was comprised of the remaining 6 groups. The ODG demonstrated a trend toward an increase in the average postvaccine dimer (0.87 ± 0.10% vs 0.67 ± 0.05%; P = .07), a significantly larger DTH response (21.5 ± 2.5 mm vs 11.3 ± 1.3 mm; P = .0002), and a trend toward decreased recurrences (3.4% vs 12.9%; P = .27). Compared with the SDG, the ODG had larger tumors (percentage ≥T2: 55% vs 23%; P = .004), more positive lymph nodes (percentage NP: 76% vs 37%; P = .001), and higher grade tumors (percentage grade 3: 52% vs 30%; P = .07), but a shorter median follow-up time (20 months vs 32 months; P <.001). CONCLUSIONS. Compared with suboptimally dosed patients, the optimally dosed E75 vaccine in disease-free BCa patients had similar toxicity but enhanced HER- 2/neu-specific immunity that may lead to decreased recurrences with additional follow-up.
AB - BACKGROUND. E75, a HER-2/neu-derived peptide, was administered as a preventive vaccine with granulocyte-macrophage-colony-stimulating factor (GM-CSF) in disease-free lymph node-positive (NP) and lymph node-negative (NN) breast cancer (BCa) patients. The optimal biologic dose (OBD) was determined based on toxicity and immunologic response. METHODS. Patients were vaccinated over 6 months (3, 4, or 6 times) with different doses of E75 plus GM-CSF. Toxicities were graded per National Cancer Institute Common Terminology Criteria. GM-CSF was reduced for significant toxicity. Immunologic response was measured by delayed type hypersensitivity test (DTH), and E75-specific CD8 + T-cells were quantified with human leukocyte antigen-A2: immunoglobulin G dimer and flow cytometry. RESULTS. Ninety-nine patients (48 NP and 51 NN) were vaccinated in 7 dose groups. The OBD was 1000 lg E75 plus 250 μg GM-CSF monthly x 6. The optimal dose group (ODG, n = 29) experienced similar toxicities to the suboptimal dose group (SDG, n = 70), which was comprised of the remaining 6 groups. The ODG demonstrated a trend toward an increase in the average postvaccine dimer (0.87 ± 0.10% vs 0.67 ± 0.05%; P = .07), a significantly larger DTH response (21.5 ± 2.5 mm vs 11.3 ± 1.3 mm; P = .0002), and a trend toward decreased recurrences (3.4% vs 12.9%; P = .27). Compared with the SDG, the ODG had larger tumors (percentage ≥T2: 55% vs 23%; P = .004), more positive lymph nodes (percentage NP: 76% vs 37%; P = .001), and higher grade tumors (percentage grade 3: 52% vs 30%; P = .07), but a shorter median follow-up time (20 months vs 32 months; P <.001). CONCLUSIONS. Compared with suboptimally dosed patients, the optimally dosed E75 vaccine in disease-free BCa patients had similar toxicity but enhanced HER- 2/neu-specific immunity that may lead to decreased recurrences with additional follow-up.
KW - Breast cancer
KW - Dosing
KW - E75
KW - Peptide
KW - Vaccine
UR - http://www.scopus.com/inward/record.url?scp=54049092448&partnerID=8YFLogxK
U2 - 10.1002/cncr.23772
DO - 10.1002/cncr.23772
M3 - Article
C2 - 18726994
AN - SCOPUS:54049092448
SN - 0008-543X
VL - 113
SP - 1666
EP - 1675
JO - Cancer
JF - Cancer
IS - 7
ER -