Orally administered nitrite attenuates cardiac allograft rejection in rats

Jianghua Zhan, Atsunori Nakao, Ryujiro Sugimoto, Rajeev Dhupar, Yinna Wang, Zhiliang Wang, Timothy R. Billiar, Kenneth R. McCurry*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


Background: Recent studies have shown that nitrite serves as an endogenous reservoir of nitric oxide (NO), particularly in the presence of hypoxia and ischemia. We hypothesized that exogenous nitrite supplementation would protect cardiac allografts. Methods: Fully allogeneic heterotopic heart transplantation was performed in LEW to BN combination under tacrolimus. Animals were given either regular or nitrite/nitrate (NOx)-low chow combined with regular water or water containing nitrite (50 mg/L) for 120 days continuously beginning 7 days before transplant. Results: Serum nitrite/nitrate levels were significantly higher in animals given nitrite water for 30 days, and lower in the animals fed with NOx-low diet than those in animals who received standard diet. Supplementation of drinking water with nitrite enhanced heart graft survival to a median of >120 days from 49.5 days in animals fed a standard diet. In contrast, dietary NOx insufficiency resulted in significantly earlier rejection of allografts (30.5 days). In correlation with graft survival, mRNA levels for interferon-γ in the spleen or tumor necrosis factor-α in the grafts were significantly less when animals were fed nitrite water compared with those without nitrite supplementation. Conclusion: These data demonstrate that dietary nitrite supplementation was significantly effective in preventing development of allograft rejection.

Original languageEnglish
Pages (from-to)155-165
Number of pages11
Issue number2
StatePublished - Aug 2009
Externally publishedYes


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