Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry

Oleg Chertov; Arya Biragyn; Larry W. Kwak; John T. Simpson; Tatiana Boronina; Van M. Hoang; Da Rue A. Prieto; Thomas P. Conrads; Timothy D. Veenstra; Robert J. Fisher

doi:10.1002/pmic.200300677

Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry

Oleg Chertov^*, Arya Biragyn, Larry W. Kwak, John T. Simpson, Tatiana Boronina, Van M. Hoang, Da Rue A. Prieto, Thomas P. Conrads, Timothy D. Veenstra, Robert J. Fisher

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

134 Scopus citations

Abstract

A method to extract peptides and low molecular weight proteins from serum under denaturing conditions using acetonitrile containing 0.1% trifluoroacetic acid has been developed. The extraction procedure precipitates large, abundant proteins to simplify subsequent mass spectral analysis. This sample preparation method provides an efficient way to extract serum peptides, enabling them to be compared and identified using different mass spectrometry approaches. Surface-enhanced laser desorption/ionization-time of flight mass spectrometry analysis of mouse blood serum samples prepared by this method allowed detection of two markers which were significantly reduced in mice with B cell lymphoma tumor. One of these markers has been identified as apolipoprotein A-II.

Original language	English
Pages (from-to)	1195-1203
Number of pages	9
Journal	Proteomics
Volume	4
Issue number	4
DOIs	https://doi.org/10.1002/pmic.200300677
State	Published - Apr 2004
Externally published	Yes

Keywords

Apolipoprotein A-II
Biomarkers
Serum
Surface-enhanced laser desorption/ionization
Tumor

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10.1002/pmic.200300677

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Chertov, O., Biragyn, A., Kwak, L. W., Simpson, J. T., Boronina, T., Hoang, V. M., Prieto, D. R. A., Conrads, T. P., Veenstra, T. D., & Fisher, R. J. (2004). Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry. Proteomics, 4(4), 1195-1203. https://doi.org/10.1002/pmic.200300677

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title = "Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry",

abstract = "A method to extract peptides and low molecular weight proteins from serum under denaturing conditions using acetonitrile containing 0.1% trifluoroacetic acid has been developed. The extraction procedure precipitates large, abundant proteins to simplify subsequent mass spectral analysis. This sample preparation method provides an efficient way to extract serum peptides, enabling them to be compared and identified using different mass spectrometry approaches. Surface-enhanced laser desorption/ionization-time of flight mass spectrometry analysis of mouse blood serum samples prepared by this method allowed detection of two markers which were significantly reduced in mice with B cell lymphoma tumor. One of these markers has been identified as apolipoprotein A-II.",

keywords = "Apolipoprotein A-II, Biomarkers, Serum, Surface-enhanced laser desorption/ionization, Tumor",

author = "Oleg Chertov and Arya Biragyn and Kwak, {Larry W.} and Simpson, {John T.} and Tatiana Boronina and Hoang, {Van M.} and Prieto, {Da Rue A.} and Conrads, {Thomas P.} and Veenstra, {Timothy D.} and Fisher, {Robert J.}",

year = "2004",

month = apr,

doi = "10.1002/pmic.200300677",

language = "English",

volume = "4",

pages = "1195--1203",

journal = "Proteomics",

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Chertov, O, Biragyn, A, Kwak, LW, Simpson, JT, Boronina, T, Hoang, VM, Prieto, DRA, Conrads, TP, Veenstra, TD & Fisher, RJ 2004, 'Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry', Proteomics, vol. 4, no. 4, pp. 1195-1203. https://doi.org/10.1002/pmic.200300677

TY - JOUR

T1 - Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry

AU - Chertov, Oleg

AU - Biragyn, Arya

AU - Kwak, Larry W.

AU - Simpson, John T.

AU - Boronina, Tatiana

AU - Hoang, Van M.

AU - Prieto, Da Rue A.

AU - Conrads, Thomas P.

AU - Veenstra, Timothy D.

AU - Fisher, Robert J.

PY - 2004/4

Y1 - 2004/4

N2 - A method to extract peptides and low molecular weight proteins from serum under denaturing conditions using acetonitrile containing 0.1% trifluoroacetic acid has been developed. The extraction procedure precipitates large, abundant proteins to simplify subsequent mass spectral analysis. This sample preparation method provides an efficient way to extract serum peptides, enabling them to be compared and identified using different mass spectrometry approaches. Surface-enhanced laser desorption/ionization-time of flight mass spectrometry analysis of mouse blood serum samples prepared by this method allowed detection of two markers which were significantly reduced in mice with B cell lymphoma tumor. One of these markers has been identified as apolipoprotein A-II.

AB - A method to extract peptides and low molecular weight proteins from serum under denaturing conditions using acetonitrile containing 0.1% trifluoroacetic acid has been developed. The extraction procedure precipitates large, abundant proteins to simplify subsequent mass spectral analysis. This sample preparation method provides an efficient way to extract serum peptides, enabling them to be compared and identified using different mass spectrometry approaches. Surface-enhanced laser desorption/ionization-time of flight mass spectrometry analysis of mouse blood serum samples prepared by this method allowed detection of two markers which were significantly reduced in mice with B cell lymphoma tumor. One of these markers has been identified as apolipoprotein A-II.

KW - Apolipoprotein A-II

KW - Biomarkers

KW - Serum

KW - Surface-enhanced laser desorption/ionization

KW - Tumor

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DO - 10.1002/pmic.200300677

M3 - Article

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AN - SCOPUS:11144353532

SN - 1615-9853

VL - 4

SP - 1195

EP - 1203

JO - Proteomics

JF - Proteomics

IS - 4

ER -

Organic solvent extraction of proteins and peptides from serum as an effective sample preparation for detection and identification of biomarkers by mass spectrometry

Abstract

Keywords

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