Osteoblast-specific Factor 2 Expression in Prostate Cancer-associated Stroma: Identification Through Microarray Technology

Bungo Furusato*, Toshiyuki Tsunoda, Syed Shaheduzzaman, Martin E. Nau, Maryanne Vahey, Gyorgy Petrovics, David G. McLeod, Seiji Naito, Senji Shirasawa, Shiv Srivastava, Isabell A. Sesterhenn

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Objectives: To better understand the gene expression patterns in tumor-associated stroma, laser-capture-microdissections from clinical specimens were analyzed by genome-wide-expression microarray technology. The epithelial-stromal interaction plays a critical role in prostate development, reactive changes, and tumorigenesis. Diverse microarray technologies have been used to characterize the molecular changes in prostate cancer. Even though these gene expression studies are compromised by the heterogeneity of the tumor, as well as by the difficulty associated with collecting appropriate counterparts to represent normal prostate cells, the gene array data from tumors have shown promising results. Currently, little is known about the tumor-associated stromal gene expression profile in prostate cancer. Methods: Matching benign and malignant epithelial cell-related stroma cells were subjected to microarray platforms. Results: The prostatatic stroma expressed several osteogenic molecules. In particular, one of the genes, OSF2, was upregulated in tumor-associated stroma compared with benign epithelial cell associated stroma, which was further validated by immunohistochemical examination. Conclusions: These data show that the combination of laser capture dissection with computational enhancement of epithelial and stromal microarray data is a useful tool to assess gene expression changes in prostate cancer stroma.

Original languageEnglish
Pages (from-to)768-772
Number of pages5
Issue number4
StatePublished - Apr 2010
Externally publishedYes


Dive into the research topics of 'Osteoblast-specific Factor 2 Expression in Prostate Cancer-associated Stroma: Identification Through Microarray Technology'. Together they form a unique fingerprint.

Cite this