Parasite Antigen-Driven Basophils Are a Major Source of IL-4 in Human Filarial Infections

Basophil contribution to the IL-4 pool in filarial infections was assessed using PBMC from 20 patients with active filarial infections and from 9 uninfected subjects. Patient basophils released histamine in response to Brugia malayi Ag (BmAg). They also released IL-4 within 2 h after exposure to BmAg, as assessed by intracellular cytokine flow cytometry. This IL-4 induction was Ag specific, as IL-4 was not detected in BmAg-exposed basophils obtained from uninfected subjects. Although there were, on average, 64 times more CD4 ⁺ T cells than basophils in the peripheral circulation of filaria-infected patients, the absolute numbers of basophils and CD4 ⁺ T cells producing IL-4 per 100,000 PBMC were equivalent (geometric mean: 16 IL-4-producing basophils/100,000 PBMC vs 22 IL-4-producing CD4 ⁺ T cells/100,000 PBMC). Basophils also released IL-4 in response to both low and high concentrations of BmAg, whereas CD4⁺ T cells released IL-4 only after incubation with a high concentration of BmAg, raising the possibility that basophils, due to their lower threshold for activation, may actually release IL-4 more frequently than CD4⁺ T cells in vivo. Furthermore, IL-4 production in vitro by Ag-stimulated purified basophils or CD4⁺ T cells provided evidence that basophils release greater quantities of IL-4 per cell than CD4⁺ T cells in response to BmAg. These results suggest that, when Ag-specific IgE is present in a filaria-infected individual, basophils function to amplify the ongoing Th2 response by releasing IL-4 in greater amounts and possibly more frequently than CD4⁺ T cells in response to filarial Ag.