PCGEM1, a prostate-specific gene, is overexpressed in prostate cancer

Vasantha Srikantan, Zhiqiang Zou, Gyorgy Petrovics, Linda Xu, Meena Augustus, Leland Davis, Jeffrey R. Livezey, Theresa Connell, Isabell A. Sesterhenn, Kiyoshi Yoshino, Gregory S. Buzard, F. K. Mostofi, David G. McLeod, Judd W. Moul, Shiv Srivastava*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

286 Scopus citations

Abstract

A prostate-specific gene, PCGEM1, was identified by differential display analysis of paired normal and prostate cancer tissues. Multiple tissue Northern blot analysis revealed that PCGEM1 was expressed exclusively in human prostate tissue. Analysis of PCGEM1 expression in matched normal and primary tumor specimens revealed tumor-associated overexpression in 84% of patients with prostate cancer by in situ hybridization assay and in 56% of patients by reverse transcription-PCR assay. Among various prostate cancer cell lines analyzed, PCGEM1 expression was detected only in the androgen receptor-positive cell line LNCaP. Extensive DNA sequence analysis of the PCGEM1 cDNA and genomic DNA revealed that PCGEM1 lacks protein-coding capacity and suggests that it may belong to an emerging class of noncoding RNAs, also called 'riboregulators.' The PCGEM1 locus was mapped to chromosome 2q32. Taken together, the remarkable prostate-tissue specificity and androgen-dependent expression of PCGEM1 as well as its elevated expression in a significant percentage of tumor tissues suggest specific functions of PCGEM1 in the biology and tumorigenesis of the prostate gland.

Original languageEnglish
Pages (from-to)12216-12221
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number22
DOIs
StatePublished - 24 Oct 2000
Externally publishedYes

Keywords

  • Androgen regulation
  • Differential display
  • Noncoding RNA
  • Riboregulator

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