TY - JOUR
T1 - Periodic re-emergence of endemic strains with strong epidemic potential-A proposed explanation for the 2004 Indonesian dengue epidemic
AU - Ong, Swee Hoe
AU - Yip, Jin Teen
AU - Chen, Yen Liang
AU - Liu, Wei
AU - Harun, Syahrial
AU - Lystiyaningsih, Erlin
AU - Heriyanto, Bambang
AU - Beckett, Charmagne G.
AU - Mitchell, Wayne P.
AU - Hibberd, Martin L.
AU - Suwandono, Agus
AU - Vasudevan, Subhash G.
AU - Schreiber, Mark J.
PY - 2008/3
Y1 - 2008/3
N2 - Indonesia experienced a severe dengue epidemic in the first quarter of 2004 with 58,301 cases and 658 deaths reported to the WHO. All four dengue virus (DENV) serotypes were detected, with DENV-3 the predominant strain. To ascertain the molecular epidemiology of the DENV associated with the epidemic, complete genomes of 15 isolates were sequenced from patient serum collected in Jakarta during the epidemic, and two historical DENV-3 isolates from previous epidemics in 1988 and 1998 were selectively sequenced for comparative studies. Phylogenetic trees for all four serotypes indicate the viruses are endemic strains that have been circulating in Indonesia for a few decades. Whole-genome phylogeny showed the 2004 DENV-3 isolates share high similarity with those isolated in 1998 during a major epidemic in Sumatra. Together these subtype I DENV-3 strains form a Sumatran-Javan clade with demonstrated epidemic potential. No newly-acquired amino acid mutations were found while comparing genomes from the two epidemics. This suggests re-emergence of little-changed endemic strains as causative agents of the epidemic in 2004. Notably, the molecular evidence rules out change in the viral genomes as the trigger of the epidemic.
AB - Indonesia experienced a severe dengue epidemic in the first quarter of 2004 with 58,301 cases and 658 deaths reported to the WHO. All four dengue virus (DENV) serotypes were detected, with DENV-3 the predominant strain. To ascertain the molecular epidemiology of the DENV associated with the epidemic, complete genomes of 15 isolates were sequenced from patient serum collected in Jakarta during the epidemic, and two historical DENV-3 isolates from previous epidemics in 1988 and 1998 were selectively sequenced for comparative studies. Phylogenetic trees for all four serotypes indicate the viruses are endemic strains that have been circulating in Indonesia for a few decades. Whole-genome phylogeny showed the 2004 DENV-3 isolates share high similarity with those isolated in 1998 during a major epidemic in Sumatra. Together these subtype I DENV-3 strains form a Sumatran-Javan clade with demonstrated epidemic potential. No newly-acquired amino acid mutations were found while comparing genomes from the two epidemics. This suggests re-emergence of little-changed endemic strains as causative agents of the epidemic in 2004. Notably, the molecular evidence rules out change in the viral genomes as the trigger of the epidemic.
KW - Dengue
KW - Epidemic
KW - Genome
KW - Molecular epidemiology
UR - http://www.scopus.com/inward/record.url?scp=39749173715&partnerID=8YFLogxK
U2 - 10.1016/j.meegid.2007.12.005
DO - 10.1016/j.meegid.2007.12.005
M3 - Article
C2 - 18243816
AN - SCOPUS:39749173715
SN - 1567-1348
VL - 8
SP - 191
EP - 204
JO - Infection, Genetics and Evolution
JF - Infection, Genetics and Evolution
IS - 2
ER -