Peripheral CD4+ T Cell cytokine responses following human challenge and Re-Challenge with campylobacter jejuni

Kelly A. Fimlaid*, Janet C. Lindow, David R. Tribble, Janice Y. Bunn, Alexander C. Maue, Beth D. Kirkpatrick

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


Campylobacter jejuni is a leading cause of human gastroenteritis worldwide; however, our understanding of the human immune response to C. jejuni infection is limited. A previous human challenge model has shown that C. jejuni elicits IFNc production by peripheral blood mononuclear cells, a response associated with protection from clinical disease following reinfection. In this study, we investigate T lymphocyte profiles associated with campylobacteriosis using specimens from a new human challenge model in which C. jejuni-na?ve subjects were challenged and re-challenged with C. jejuni CG8421. Multiparameter flow cytometry was used to investigate T lymphocytes as a source of cytokines, including IFNc, and to identify cytokine patterns associated with either campylobacteriosis or protection from disease. Unexpectedly, all but one subject evaluated re-experienced campylobacteriosis after re-challenge. We show that CD4+ T cells make IFNγ and other pro-inflammatory cytokines in response to infection; however, multifunctional cytokine response patterns were not found. Cytokine production from peripheral CD4+ T cells was not enhanced following re-challenge, which may suggest deletion or tolerance. Evaluation of alternative paradigms or models is needed to better understand the immune components of protection from campylobacteriosis.

Original languageEnglish
Article numbere112513
JournalPLoS ONE
Issue number11
StatePublished - 14 Nov 2014
Externally publishedYes


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