Pertussis toxin stimulates delayed-onset, Ca2+-dependent catecholamine release and the ADP-ribosylation of a 40 kDa protein in bovine adrenal chromaffin cells

Keith W. Brocklehurst; Harvey B. Pollard

doi:10.1016/0014-5793(88)80133-9

Pertussis toxin stimulates delayed-onset, Ca²⁺-dependent catecholamine release and the ADP-ribosylation of a 40 kDa protein in bovine adrenal chromaffin cells

Keith W. Brocklehurst^*
, Harvey B. Pollard

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Pertussis toxin was found to stimulate catecholamine release from bovine adrenal chromaffin cells in a Ca²⁺ -dependent manner and in the absence of any stimulatory or inhibitory agonists for this cell. The release of catecholamine was associated with the ADP-ribosylation of an approx. 40 kDa protein present in the total membrane fraction. These results are consistent with the existence of an exocytosis-linked G-protein.

Original language	English
Pages (from-to)	439-445
Number of pages	7
Journal	FEBS Letters
Volume	234
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(88)80133-9
State	Published - 18 Jul 1988

Keywords

(Adrenal medulla)
ADP-ribosylation
Exocytosis
G-protein
Pertussis toxin
Stimulus-secretion coupling

Access to Document

10.1016/0014-5793(88)80133-9

Cite this

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title = "Pertussis toxin stimulates delayed-onset, Ca2+-dependent catecholamine release and the ADP-ribosylation of a 40 kDa protein in bovine adrenal chromaffin cells",

abstract = "Pertussis toxin was found to stimulate catecholamine release from bovine adrenal chromaffin cells in a Ca2+ -dependent manner and in the absence of any stimulatory or inhibitory agonists for this cell. The release of catecholamine was associated with the ADP-ribosylation of an approx. 40 kDa protein present in the total membrane fraction. These results are consistent with the existence of an exocytosis-linked G-protein.",

keywords = "(Adrenal medulla), ADP-ribosylation, Exocytosis, G-protein, Pertussis toxin, Stimulus-secretion coupling",

author = "Brocklehurst, \{Keith W.\} and Pollard, \{Harvey B.\}",

year = "1988",

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T1 - Pertussis toxin stimulates delayed-onset, Ca2+-dependent catecholamine release and the ADP-ribosylation of a 40 kDa protein in bovine adrenal chromaffin cells

AU - Brocklehurst, Keith W.

AU - Pollard, Harvey B.

PY - 1988/7/18

Y1 - 1988/7/18

N2 - Pertussis toxin was found to stimulate catecholamine release from bovine adrenal chromaffin cells in a Ca2+ -dependent manner and in the absence of any stimulatory or inhibitory agonists for this cell. The release of catecholamine was associated with the ADP-ribosylation of an approx. 40 kDa protein present in the total membrane fraction. These results are consistent with the existence of an exocytosis-linked G-protein.

AB - Pertussis toxin was found to stimulate catecholamine release from bovine adrenal chromaffin cells in a Ca2+ -dependent manner and in the absence of any stimulatory or inhibitory agonists for this cell. The release of catecholamine was associated with the ADP-ribosylation of an approx. 40 kDa protein present in the total membrane fraction. These results are consistent with the existence of an exocytosis-linked G-protein.

KW - (Adrenal medulla)

KW - ADP-ribosylation

KW - Exocytosis

KW - G-protein

KW - Pertussis toxin

KW - Stimulus-secretion coupling

UR - https://www.scopus.com/pages/publications/0024291247

U2 - 10.1016/0014-5793(88)80133-9

DO - 10.1016/0014-5793(88)80133-9

M3 - Article

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SN - 0014-5793

VL - 234

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EP - 445

JO - FEBS Letters

JF - FEBS Letters

IS - 2