Phosphoinositide turnover in human platelets is stimulated by albumin

Riyad M. Amin; James H. Hays; K. M. Mohamed Shakir

doi:10.1016/0024-3205(91)90313-Z

Phosphoinositide turnover in human platelets is stimulated by albumin

Riyad M. Amin, James H. Hays, K. M. Mohamed Shakir^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The effect of human serum albumin (HSA) on the hydrolysis of phosphatidylinositides in human platelets labeled with myo(³H)inositol was studied. Incubation of platelets with HSA (4 gm/dl) for 10 seconds increased IP₂ and IP₃ by 169% and 217% respectively. 93% of IP₃ accumulated within the first 10 seconds. This effect was also shared by bovine serum albumin, although no changes in IP₃ levels occured with ovalbumin. All albumin species used induced ⁴⁵Ca⁺² release from platelets irrespective of its effect on IP₃ accumulation. These findings indicate that albumin may function in biological systems by inducing intracellular signaling.

Original language	English
Pages (from-to)	1707-1719
Number of pages	13
Journal	Life Sciences
Volume	49
Issue number	23
DOIs	https://doi.org/10.1016/0024-3205(91)90313-Z
State	Published - 1991
Externally published	Yes

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title = "Phosphoinositide turnover in human platelets is stimulated by albumin",

abstract = "The effect of human serum albumin (HSA) on the hydrolysis of phosphatidylinositides in human platelets labeled with myo(3H)inositol was studied. Incubation of platelets with HSA (4 gm/dl) for 10 seconds increased IP2 and IP3 by 169% and 217% respectively. 93% of IP3 accumulated within the first 10 seconds. This effect was also shared by bovine serum albumin, although no changes in IP3 levels occured with ovalbumin. All albumin species used induced 45Ca+2 release from platelets irrespective of its effect on IP3 accumulation. These findings indicate that albumin may function in biological systems by inducing intracellular signaling.",

author = "Amin, {Riyad M.} and Hays, {James H.} and {Mohamed Shakir}, {K. M.}",

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T1 - Phosphoinositide turnover in human platelets is stimulated by albumin

AU - Amin, Riyad M.

AU - Hays, James H.

AU - Mohamed Shakir, K. M.

PY - 1991

Y1 - 1991

N2 - The effect of human serum albumin (HSA) on the hydrolysis of phosphatidylinositides in human platelets labeled with myo(3H)inositol was studied. Incubation of platelets with HSA (4 gm/dl) for 10 seconds increased IP2 and IP3 by 169% and 217% respectively. 93% of IP3 accumulated within the first 10 seconds. This effect was also shared by bovine serum albumin, although no changes in IP3 levels occured with ovalbumin. All albumin species used induced 45Ca+2 release from platelets irrespective of its effect on IP3 accumulation. These findings indicate that albumin may function in biological systems by inducing intracellular signaling.

AB - The effect of human serum albumin (HSA) on the hydrolysis of phosphatidylinositides in human platelets labeled with myo(3H)inositol was studied. Incubation of platelets with HSA (4 gm/dl) for 10 seconds increased IP2 and IP3 by 169% and 217% respectively. 93% of IP3 accumulated within the first 10 seconds. This effect was also shared by bovine serum albumin, although no changes in IP3 levels occured with ovalbumin. All albumin species used induced 45Ca+2 release from platelets irrespective of its effect on IP3 accumulation. These findings indicate that albumin may function in biological systems by inducing intracellular signaling.

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U2 - 10.1016/0024-3205(91)90313-Z

DO - 10.1016/0024-3205(91)90313-Z

M3 - Article

C2 - 1658519

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VL - 49

SP - 1707

EP - 1719

JO - Life Sciences

JF - Life Sciences

IS - 23

ER -

Phosphoinositide turnover in human platelets is stimulated by albumin

Abstract

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