Phosphoproteomic analysis of pancreatic ductal adenocarcinoma cells reveals differential phosphorylation of cell adhesion, cell junction and structural proteins

Weidong Zhou; Michela Capello; Claudia Fredolini; Leda Racanicchi; Lorenzo Piemonti; Lance A. Liotta; Francesco Novelli; Emanuel F. Petricoin

doi:10.4172/jpb.1000186

Phosphoproteomic analysis of pancreatic ductal adenocarcinoma cells reveals differential phosphorylation of cell adhesion, cell junction and structural proteins

Weidong Zhou^*, Michela Capello, Claudia Fredolini, Leda Racanicchi, Lorenzo Piemonti, Lance A. Liotta, Francesco Novelli, Emanuel F. Petricoin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

In this present work, we characterized the phosphoproteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap. We identified more than 700 phosphoproteins from each sample, and revealed differential phosphorylation of many proteins involved in cell adhesion, cell junction, and cytoskeleton. Since post-translational phosphorylation is a common and important mechanism of acute and reversible regulation of protein function in mammalian cells, an understanding of differential phosphorylation of these proteins and resulting signal transduction changes in PDAC will help in comprehending the complex dynamics of tumor invasion and metastasis in pancreatic cancer.

Original language	English
Pages (from-to)	170-178
Number of pages	9
Journal	Journal of Proteomics and Bioinformatics
Volume	4
Issue number	9
DOIs	https://doi.org/10.4172/jpb.1000186
State	Published - Sep 2011
Externally published	Yes

Keywords

Cell adhesion
Cell junction
Cytoskeleton
LTQ-orbitrap
Mass spectrometry
Pancreatic ductal adenocarcinoma
Phosphoproteomics

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10.4172/jpb.1000186

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Zhou, W., Capello, M., Fredolini, C., Racanicchi, L., Piemonti, L., Liotta, L. A., Novelli, F., & Petricoin, E. F. (2011). Phosphoproteomic analysis of pancreatic ductal adenocarcinoma cells reveals differential phosphorylation of cell adhesion, cell junction and structural proteins. Journal of Proteomics and Bioinformatics, 4(9), 170-178. https://doi.org/10.4172/jpb.1000186

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title = "Phosphoproteomic analysis of pancreatic ductal adenocarcinoma cells reveals differential phosphorylation of cell adhesion, cell junction and structural proteins",

abstract = "In this present work, we characterized the phosphoproteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap. We identified more than 700 phosphoproteins from each sample, and revealed differential phosphorylation of many proteins involved in cell adhesion, cell junction, and cytoskeleton. Since post-translational phosphorylation is a common and important mechanism of acute and reversible regulation of protein function in mammalian cells, an understanding of differential phosphorylation of these proteins and resulting signal transduction changes in PDAC will help in comprehending the complex dynamics of tumor invasion and metastasis in pancreatic cancer.",

keywords = "Cell adhesion, Cell junction, Cytoskeleton, LTQ-orbitrap, Mass spectrometry, Pancreatic ductal adenocarcinoma, Phosphoproteomics",

author = "Weidong Zhou and Michela Capello and Claudia Fredolini and Leda Racanicchi and Lorenzo Piemonti and Liotta, {Lance A.} and Francesco Novelli and Petricoin, {Emanuel F.}",

year = "2011",

month = sep,

doi = "10.4172/jpb.1000186",

language = "English",

volume = "4",

pages = "170--178",

journal = "Journal of Proteomics and Bioinformatics",

issn = "0974-276X",

number = "9",

}

Zhou, W, Capello, M, Fredolini, C, Racanicchi, L, Piemonti, L, Liotta, LA, Novelli, F & Petricoin, EF 2011, 'Phosphoproteomic analysis of pancreatic ductal adenocarcinoma cells reveals differential phosphorylation of cell adhesion, cell junction and structural proteins', Journal of Proteomics and Bioinformatics, vol. 4, no. 9, pp. 170-178. https://doi.org/10.4172/jpb.1000186

Phosphoproteomic analysis of pancreatic ductal adenocarcinoma cells reveals differential phosphorylation of cell adhesion, cell junction and structural proteins. / Zhou, Weidong; Capello, Michela; Fredolini, Claudia et al.
In: Journal of Proteomics and Bioinformatics, Vol. 4, No. 9, 09.2011, p. 170-178.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Phosphoproteomic analysis of pancreatic ductal adenocarcinoma cells reveals differential phosphorylation of cell adhesion, cell junction and structural proteins

AU - Zhou, Weidong

AU - Capello, Michela

AU - Fredolini, Claudia

AU - Racanicchi, Leda

AU - Piemonti, Lorenzo

AU - Liotta, Lance A.

AU - Novelli, Francesco

AU - Petricoin, Emanuel F.

PY - 2011/9

Y1 - 2011/9

N2 - In this present work, we characterized the phosphoproteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap. We identified more than 700 phosphoproteins from each sample, and revealed differential phosphorylation of many proteins involved in cell adhesion, cell junction, and cytoskeleton. Since post-translational phosphorylation is a common and important mechanism of acute and reversible regulation of protein function in mammalian cells, an understanding of differential phosphorylation of these proteins and resulting signal transduction changes in PDAC will help in comprehending the complex dynamics of tumor invasion and metastasis in pancreatic cancer.

AB - In this present work, we characterized the phosphoproteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap. We identified more than 700 phosphoproteins from each sample, and revealed differential phosphorylation of many proteins involved in cell adhesion, cell junction, and cytoskeleton. Since post-translational phosphorylation is a common and important mechanism of acute and reversible regulation of protein function in mammalian cells, an understanding of differential phosphorylation of these proteins and resulting signal transduction changes in PDAC will help in comprehending the complex dynamics of tumor invasion and metastasis in pancreatic cancer.

KW - Cell adhesion

KW - Cell junction

KW - Cytoskeleton

KW - LTQ-orbitrap

KW - Mass spectrometry

KW - Pancreatic ductal adenocarcinoma

KW - Phosphoproteomics

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DO - 10.4172/jpb.1000186

M3 - Article

AN - SCOPUS:84861765345

SN - 0974-276X

VL - 4

SP - 170

EP - 178

JO - Journal of Proteomics and Bioinformatics

JF - Journal of Proteomics and Bioinformatics

IS - 9

ER -

Phosphoproteomic analysis of pancreatic ductal adenocarcinoma cells reveals differential phosphorylation of cell adhesion, cell junction and structural proteins

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Keywords

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