Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

Douglas I. Walker; Kurt D. Pennell; Karan Uppal; Xiaoyan Xia; Philip K. Hopke; Mark J. Utell; Richard P. Phipps; Patricia J. Sime; Patricia Rohrbeck; Timothy M. Mallon; Dean P. Jones

doi:10.1097/JOM.0000000000000772

Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

Douglas I. Walker^*, Kurt D. Pennell, Karan Uppal, Xiaoyan Xia, Philip K. Hopke, Mark J. Utell, Richard P. Phipps, Patricia J. Sime, Patricia Rohrbeck, Timothy M. Mallon, Dean P. Jones

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.

Original language	English
Pages (from-to)	S44-S52
Journal	Journal of Occupational and Environmental Medicine
Volume	58
Issue number	8S
DOIs	https://doi.org/10.1097/JOM.0000000000000772
State	Published - 1 Aug 2016
Externally published	Yes

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@article{9e47e5470a254bdaba1321ac8ef16e62,

title = "Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel",

abstract = "Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.",

author = "Walker, {Douglas I.} and Pennell, {Kurt D.} and Karan Uppal and Xiaoyan Xia and Hopke, {Philip K.} and Utell, {Mark J.} and Phipps, {Richard P.} and Sime, {Patricia J.} and Patricia Rohrbeck and Mallon, {Timothy M.} and Jones, {Dean P.}",

note = "Publisher Copyright: {\textcopyright} 2016 American College of Occupational and Environmental Medicine.",

year = "2016",

month = aug,

day = "1",

doi = "10.1097/JOM.0000000000000772",

language = "English",

volume = "58",

pages = "S44--S52",

journal = "Journal of Occupational and Environmental Medicine",

issn = "1076-2752",

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TY - JOUR

T1 - Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

AU - Walker, Douglas I.

AU - Pennell, Kurt D.

AU - Uppal, Karan

AU - Xia, Xiaoyan

AU - Hopke, Philip K.

AU - Utell, Mark J.

AU - Phipps, Richard P.

AU - Sime, Patricia J.

AU - Rohrbeck, Patricia

AU - Mallon, Timothy M.

AU - Jones, Dean P.

PY - 2016/8/1

Y1 - 2016/8/1

N2 - Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.

AB - Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.

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DO - 10.1097/JOM.0000000000000772

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SN - 1076-2752

VL - 58

SP - S44-S52

JO - Journal of Occupational and Environmental Medicine

JF - Journal of Occupational and Environmental Medicine

IS - 8S

ER -

Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

Abstract

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