Pilot metabolome-wide association study of Benzo(a)pyrene in serum from military personnel

Douglas I. Walker*, Kurt D. Pennell, Karan Uppal, Xiaoyan Xia, Philip K. Hopke, Mark J. Utell, Richard P. Phipps, Patricia J. Sime, Patricia Rohrbeck, Timothy M. Mallon, Dean P. Jones

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


Objective: A pilot study was conducted to test the feasibility of using Department of Defense Serum Repository (DoDSR) samples to study health and exposure-related effects. Methods: Thirty unidentified human serum samples were obtained from the DoDSR and analyzed for normal serum metabolites with high-resolution mass spectrometry and serum levels of free benzo(a)pyrene (BaP) by gas chromatography-mass spectrometry. Metabolic associations with BaP were determined using a metabolome-wide association study (MWAS) and metabolic pathway enrichment. Results: The serum analysis detected normal ranges of glucose, selected amino acids, fatty acids, and creatinine. Free BaP was detected in a broad concentration range. MWAS of BaP showed associations with lipids, fatty acids, and sulfur amino acid metabolic pathways. Conclusion: The results show that the DoDSR samples are of sufficient quality for chemical profiling of DoD personnel.

Original languageEnglish
Pages (from-to)S44-S52
JournalJournal of Occupational and Environmental Medicine
Issue number8S
StatePublished - 1 Aug 2016
Externally publishedYes


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