TY - JOUR
T1 - Plasmodium falciparum phenotypic and genotypic resistance profile during the emergence of Piperaquine resistance in Northeastern Thailand
AU - Boonyalai, Nonlawat
AU - Thamnurak, Chatchadaporn
AU - Sai-ngam, Piyaporn
AU - Ta-aksorn, Winita
AU - Arsanok, Montri
AU - Uthaimongkol, Nichapat
AU - Sundrakes, Siratchana
AU - Chattrakarn, Sorayut
AU - Chaisatit, Chaiyaporn
AU - Praditpol, Chantida
AU - Fagnark, Watcharintorn
AU - Kirativanich, Kirakarn
AU - Chaorattanakawee, Suwanna
AU - Vanachayangkul, Pattaraporn
AU - Lertsethtakarn, Paphavee
AU - Gosi, Panita
AU - Utainnam, Darunee
AU - Rodkvamtook, Wuttikon
AU - Kuntawunginn, Worachet
AU - Vesely, Brian A.
AU - Spring, Michele D.
AU - Fukuda, Mark M.
AU - Lanteri, Charlotte
AU - Walsh, Douglas
AU - Saunders, David L.
AU - Smith, Philip L.
AU - Wojnarski, Mariusz
AU - Sirisopana, Narongrid
AU - Waters, Norman C.
AU - Jongsakul, Krisada
AU - Gaywee, Jariyanart
N1 - Publisher Copyright:
© 2021, The Author(s).
PY - 2021/12
Y1 - 2021/12
N2 - Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Cambodia border during 2013–2015. The majority of parasites harbored a pfmdr1-Y184F mutation. A single pfmdr1 copy number had CVIET haplotype of amino acids 72–76 of pfcrt and no pfcytb mutations. All isolates had a single pfk13 point mutation (R539T, R539I, or C580Y), and increased % survival in the ring-stage survival assay (except for R539I). Multiple copies of pfpm2 and pfcrt-F145I were detected in 2014 (12.8%) and increased to 30.4% in 2015. Parasites containing either multiple pfpm2 copies with and without pfcrt-F145I or a single pfpm2 copy with pfcrt-F145I exhibited elevated IC90 values of piperaquine. Collectively, the emergence of these resistance patterns in Thailand near Cambodia border mirrored the reports of dihydroartemisinin-piperaquine treatment failures in the adjacent province of Cambodia, Oddar Meanchey, suggesting a migration of parasites across the border. As malaria elimination efforts ramp up in Southeast Asia, host nations militaries and other groups in border regions need to coordinate the proposed interventions.
AB - Malaria remains a public health problem in Thailand, especially along its borders where highly mobile populations can contribute to persistent transmission. This study aimed to determine resistant genotypes and phenotypes of 112 Plasmodium falciparum isolates from patients along the Thai-Cambodia border during 2013–2015. The majority of parasites harbored a pfmdr1-Y184F mutation. A single pfmdr1 copy number had CVIET haplotype of amino acids 72–76 of pfcrt and no pfcytb mutations. All isolates had a single pfk13 point mutation (R539T, R539I, or C580Y), and increased % survival in the ring-stage survival assay (except for R539I). Multiple copies of pfpm2 and pfcrt-F145I were detected in 2014 (12.8%) and increased to 30.4% in 2015. Parasites containing either multiple pfpm2 copies with and without pfcrt-F145I or a single pfpm2 copy with pfcrt-F145I exhibited elevated IC90 values of piperaquine. Collectively, the emergence of these resistance patterns in Thailand near Cambodia border mirrored the reports of dihydroartemisinin-piperaquine treatment failures in the adjacent province of Cambodia, Oddar Meanchey, suggesting a migration of parasites across the border. As malaria elimination efforts ramp up in Southeast Asia, host nations militaries and other groups in border regions need to coordinate the proposed interventions.
UR - http://www.scopus.com/inward/record.url?scp=85109727112&partnerID=8YFLogxK
U2 - 10.1038/s41598-021-92735-6
DO - 10.1038/s41598-021-92735-6
M3 - Article
C2 - 34183715
AN - SCOPUS:85109727112
SN - 2045-2322
VL - 11
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 13419
ER -