TY - JOUR
T1 - Plasmonic Nanoprobe-Enabled SERS Detection of SARS-CoV-2 Proteins and Virus Samples on Wax-Printed Paper Substrates
AU - Gordon, Justine
AU - Mousavi, Seyed Danial
AU - Li, Jing
AU - Filippone, Nina
AU - Walter, Lindsey
AU - Cheng, Han Wen
AU - Skeete, Zakiya
AU - Feldman, Hallie
AU - Hakimi, Sydney
AU - Cappuccio, Kaylee
AU - Wang, Shan
AU - Hader, Melissa
AU - Shang, Guojun
AU - Turner, James
AU - Cameron, Andrew
AU - Bane, Susan
AU - Poliks, Mark
AU - Lu, Susan
AU - Yuan, Tony T.
AU - Zhong, Chuan Jian
N1 - Publisher Copyright:
© 2025 American Chemical Society
PY - 2025/10/7
Y1 - 2025/10/7
N2 - The development of highly sensitive detection methodologies for infectious diseases has become increasingly important, considering the recent COVID-19 pandemic. In this report, we demonstrate the use of a gold nanoprobe-based surface-enhanced Raman scattering (SERS) technique as a biosensor for the sensitive detection of SARS-CoV-2, including spike proteins, nucleocapsid proteins, as well as original or heat-inactivated patient samples. The nanoprobes are labeled with Raman reporter molecules for diagnostic detection and quantitative analysis of the protein or virus concentrations in the samples. Key to the detection is the capture of the targeted biomolecules by specific antibody-conjugated gold nanoparticles in a high-surface-area fibrous paper substrate, which facilitates the formation of “hot spots” for SERS signal amplification. The relationships between the SERS intensity and sample concentration were determined to assess diagnostic sensitivity and limits of detection. The results were also validated using the polymerase chain reaction (PCR) cycle threshold (CT) value vs the concentration of SARS-CoV-2. The nanoprobe-based and fibrous paper-assisted approach demonstrated a low-cost and portable platform technology for enhanced SERS detection of SARS-CoV-2, showing promise for expansion to the detection of various viruses.
AB - The development of highly sensitive detection methodologies for infectious diseases has become increasingly important, considering the recent COVID-19 pandemic. In this report, we demonstrate the use of a gold nanoprobe-based surface-enhanced Raman scattering (SERS) technique as a biosensor for the sensitive detection of SARS-CoV-2, including spike proteins, nucleocapsid proteins, as well as original or heat-inactivated patient samples. The nanoprobes are labeled with Raman reporter molecules for diagnostic detection and quantitative analysis of the protein or virus concentrations in the samples. Key to the detection is the capture of the targeted biomolecules by specific antibody-conjugated gold nanoparticles in a high-surface-area fibrous paper substrate, which facilitates the formation of “hot spots” for SERS signal amplification. The relationships between the SERS intensity and sample concentration were determined to assess diagnostic sensitivity and limits of detection. The results were also validated using the polymerase chain reaction (PCR) cycle threshold (CT) value vs the concentration of SARS-CoV-2. The nanoprobe-based and fibrous paper-assisted approach demonstrated a low-cost and portable platform technology for enhanced SERS detection of SARS-CoV-2, showing promise for expansion to the detection of various viruses.
UR - http://www.scopus.com/inward/record.url?scp=105018458910&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.5c02355
DO - 10.1021/acs.analchem.5c02355
M3 - Article
AN - SCOPUS:105018458910
SN - 0003-2700
VL - 97
SP - 21303
EP - 21313
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 39
ER -