Preclinical testing of a peptide-based, HER2/neu vaccine for prostate cancer.

Michael M. Woll*, Matthew T. Hueman, Gayle B. Ryan, Constantin G. Ioannides, Charles G. Henderson, Isabelle A. Sesterhan, Shiv Shrivasta, David G. McLeod, Judd W. Moul, George E. Peoples

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The HER2/neu protein is over-expressed in multiple epithelial tumors and the source of immunogenic peptides currently under investigation in vaccine trials in ovarian and breast cancers. We sought to define the correlation between HER2/neu expression and risk for prostate cancer recurrence and then determine the potential efficacy of anti-HER2/neu vaccination in prostate cancer patients at risk for recurrence. The risk for prostate-specific antigen (PSA) recurrence in 95 patients undergoing prostatectomy at the Walter Reed Army Medical Center (WRAMC) was calculated and correlated to HER2/neu expression, as determined by immunohistochemical staining. Peripheral blood lymphocytes (PBL) were then isolated from six consecutive human leukocyte antigen (HLA) A2+ patients with HER2/neu+ prostate tumors. These PBL were grown in parallel cultures and stimulated either with no peptide, HER2/neu E75 peptide, or control peptide. The cultures were compared for stimulated proliferation, induced peptide-specific cytotoxicity and tumor-specific cytotoxicity. When assessed by risk group, 69% of the high risk patients' tumors over-expressed HER2/neu compared to 47% of the intermediate risk group (p<0.05). Evaluation of the in vitro immune response of PBL isolated from six consecutive prostate cancer patients revealed a statistically significant increase in E75-stimulated lymphocytic proliferation. E75-stimulated lymphocytes demonstrated an E75-specific cytolytic response in 6/6 prostate cancer patients that increased with successive stimulations. Moreover, these E75-specific lymphocytes also demonstrated tumor-specific lysis against HER2/neu-expressing prostate cancer cell lines. The majority of prostate cancer patients at high risk for recurrence have HER2/neu expressing tumors. Hence, HER2/neu is a viable target for immunotherapeutics such as preventative immunization strategies with HER2/neu peptide vaccines.

Original languageEnglish
Pages (from-to)1769-1780
Number of pages12
JournalInternational journal of oncology
Issue number6
StatePublished - Dec 2004
Externally publishedYes


Dive into the research topics of 'Preclinical testing of a peptide-based, HER2/neu vaccine for prostate cancer.'. Together they form a unique fingerprint.

Cite this