Preparation and characterization of a biologic scaffold and hydrogel derived from colonic mucosa

Timothy J. Keane, Jenna Dziki, Arthur Castelton, Denver M. Faulk, Victoria Messerschmidt, Ricardo Londono, Janet E. Reing, Sachin S. Velankar, Stephen F. Badylak*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

38 Scopus citations


Gastrointestinal pathologies, injuries, and defects affect millions of individuals each year. While there are diverse treatment options for these individuals, no ideal solution exists. The repair or replacement of gastrointestinal tissue, therefore, represents a large unmet clinical need. Biomaterials derived from extracellular matrix (ECM) scaffolds have been effectively used to repair or replace numerous tissues throughout the body in both preclinical and clinical studies. Such scaffolds are prepared from decellularized tissues, and the biochemical, structural, and biologic properties vary depending upon the source tissue from which the ECM is derived. Given the potential benefit of a site-specific ECM scaffold for some applications, the objective of this study was to prepare, characterize, and determine the in vitro and in vivo cell response to ECM derived from porcine colon. Results of this study show that porcine colon can be effectively decellularized while retaining biochemical and structural constituents of the source tissue. Two forms of colonic ECM, scaffold and hydrogel, were shown to be cell friendly and facilitate the polarization of macrophages toward an M2 phenotype both in vitro and in vivo.

Original languageEnglish
Pages (from-to)291-306
Number of pages16
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number2
StatePublished - 1 Feb 2017
Externally publishedYes


  • extracellular matrix
  • hydrogel
  • regenerative medicine
  • scaffolds


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