Preparation of cardiac extracellular matrix from an intact porcine heart

John M. Wainwright, Caitlin A. Czajka, Urvi B. Patel, Donald O. Freytes, Kimimasa Tobita, Thomas W. Gilbert, Stephen F. Badylak

Research output: Contribution to journalArticlepeer-review

274 Scopus citations


Whole organ engineering would benefit from a three-dimensional scaffold produced from intact organ-specific extracellular matrix (ECM). The microenvironment and architecture provided by such a scaffold would likely support site-appropriate cell differentiation and spatial organization. The methods to produce such scaffolds from intact organs require customized decellularization protocols. In the present study, intact adult porcine hearts were successfully decellularized in less than 10h using pulsatile retrograde aortic perfusion. Serial perfusion of an enzymatic, nonionic detergent, ionic detergent, and acid solution with hypotonic and hypertonic rinses was used to systematically remove cellular content. The resultant cardiac ECM retained collagen, elastin, and glycosaminoglycans, and mechanical integrity. Cardiac ECM supported the formation of organized chicken cardiomyocyte sarcomere structure in vitro. The intact decellularized porcine heart provides a tissue engineering template that may be beneficial for future preclinical studies and eventual clinical applications.

Original languageEnglish
Pages (from-to)525-532
Number of pages8
JournalTissue Engineering - Part C: Methods
Issue number3
StatePublished - 1 Jun 2010
Externally publishedYes


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