TY - JOUR
T1 - Preparative isoelectric focusing of human serum very low-density and low-density lipoproteins
AU - Sundaram, Shanmuga G.
AU - Mohamed Shakir, K. M.
AU - Margolis, Simeon
N1 - Funding Information:
This work was supported by Grant No. HL 15930 from the National Institutes of Health, United States Public Health Service. The technical assistance of Ronald Jones is gratefully acknowledged.
PY - 1978/8/1
Y1 - 1978/8/1
N2 - Ten percent glycerol prevented the usual precipitation of human serum very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) at their isoelectric points during their preparative isoelectric focusing (IEF), IEF separated VLDL and LDL into two major fractions. The observed optical density peaks are not artifacts caused by binding of Ampholines to VLDL or LDL since no radioactivity accumulated in the fractions containing VLDL or LDL during IEF in the presence of [14C]Ampholine, and gel filtration completely separated the lipoproteins from [14C]Ampholine. These results suggest that IEF may separate subspecies of VLDL and LDL under suitable experimental conditions.
AB - Ten percent glycerol prevented the usual precipitation of human serum very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) at their isoelectric points during their preparative isoelectric focusing (IEF), IEF separated VLDL and LDL into two major fractions. The observed optical density peaks are not artifacts caused by binding of Ampholines to VLDL or LDL since no radioactivity accumulated in the fractions containing VLDL or LDL during IEF in the presence of [14C]Ampholine, and gel filtration completely separated the lipoproteins from [14C]Ampholine. These results suggest that IEF may separate subspecies of VLDL and LDL under suitable experimental conditions.
UR - http://www.scopus.com/inward/record.url?scp=0018127593&partnerID=8YFLogxK
U2 - 10.1016/0003-2697(78)90440-2
DO - 10.1016/0003-2697(78)90440-2
M3 - Article
C2 - 211872
AN - SCOPUS:0018127593
SN - 0003-2697
VL - 88
SP - 425
EP - 433
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -