Preparative isoelectric focusing of human serum very low-density and low-density lipoproteins

Shanmuga G. Sundaram; K. M. Mohamed Shakir; Simeon Margolis

doi:10.1016/0003-2697(78)90440-2

Preparative isoelectric focusing of human serum very low-density and low-density lipoproteins

Shanmuga G. Sundaram^*, K. M. Mohamed Shakir, Simeon Margolis

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Ten percent glycerol prevented the usual precipitation of human serum very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) at their isoelectric points during their preparative isoelectric focusing (IEF), IEF separated VLDL and LDL into two major fractions. The observed optical density peaks are not artifacts caused by binding of Ampholines to VLDL or LDL since no radioactivity accumulated in the fractions containing VLDL or LDL during IEF in the presence of [¹⁴C]Ampholine, and gel filtration completely separated the lipoproteins from [¹⁴C]Ampholine. These results suggest that IEF may separate subspecies of VLDL and LDL under suitable experimental conditions.

Original language	English
Pages (from-to)	425-433
Number of pages	9
Journal	Analytical Biochemistry
Volume	88
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(78)90440-2
State	Published - 1 Aug 1978
Externally published	Yes

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title = "Preparative isoelectric focusing of human serum very low-density and low-density lipoproteins",

abstract = "Ten percent glycerol prevented the usual precipitation of human serum very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) at their isoelectric points during their preparative isoelectric focusing (IEF), IEF separated VLDL and LDL into two major fractions. The observed optical density peaks are not artifacts caused by binding of Ampholines to VLDL or LDL since no radioactivity accumulated in the fractions containing VLDL or LDL during IEF in the presence of [14C]Ampholine, and gel filtration completely separated the lipoproteins from [14C]Ampholine. These results suggest that IEF may separate subspecies of VLDL and LDL under suitable experimental conditions.",

author = "Sundaram, {Shanmuga G.} and {Mohamed Shakir}, {K. M.} and Simeon Margolis",

note = "Funding Information: This work was supported by Grant No. HL 15930 from the National Institutes of Health, United States Public Health Service. The technical assistance of Ronald Jones is gratefully acknowledged.",

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T1 - Preparative isoelectric focusing of human serum very low-density and low-density lipoproteins

AU - Sundaram, Shanmuga G.

AU - Mohamed Shakir, K. M.

AU - Margolis, Simeon

N1 - Funding Information: This work was supported by Grant No. HL 15930 from the National Institutes of Health, United States Public Health Service. The technical assistance of Ronald Jones is gratefully acknowledged.

PY - 1978/8/1

Y1 - 1978/8/1

N2 - Ten percent glycerol prevented the usual precipitation of human serum very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) at their isoelectric points during their preparative isoelectric focusing (IEF), IEF separated VLDL and LDL into two major fractions. The observed optical density peaks are not artifacts caused by binding of Ampholines to VLDL or LDL since no radioactivity accumulated in the fractions containing VLDL or LDL during IEF in the presence of [14C]Ampholine, and gel filtration completely separated the lipoproteins from [14C]Ampholine. These results suggest that IEF may separate subspecies of VLDL and LDL under suitable experimental conditions.

AB - Ten percent glycerol prevented the usual precipitation of human serum very low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) at their isoelectric points during their preparative isoelectric focusing (IEF), IEF separated VLDL and LDL into two major fractions. The observed optical density peaks are not artifacts caused by binding of Ampholines to VLDL or LDL since no radioactivity accumulated in the fractions containing VLDL or LDL during IEF in the presence of [14C]Ampholine, and gel filtration completely separated the lipoproteins from [14C]Ampholine. These results suggest that IEF may separate subspecies of VLDL and LDL under suitable experimental conditions.

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DO - 10.1016/0003-2697(78)90440-2

M3 - Article

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SN - 0003-2697

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JO - Analytical Biochemistry

JF - Analytical Biochemistry

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ER -

Preparative isoelectric focusing of human serum very low-density and low-density lipoproteins

Abstract

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